Effects of Exogenous NO on Soil Nutrient Content and Seedling Growth Characteristics from Potted Catharanthus roseus under Light-shading Stress

doi:10.7525/j.issn.1673-5102.2022.06.019

Abstract

Abstract:

To explore the effect of exogenous NO donor（sodium nitroprusside， SNP） on soil nutrient content and Catharanthus roseus seedlings growth under light-shading stress， four treatments（normal culture， external application of SNP， light-shading treatment and external application of SNP+ shading treatment） were designed， and the change characteristics of C， N， P nutrient contents and physicochemical index from potted soil， and the seedling shoot growth index（plant height， leaf length， leaf width， leaf area， fresh mass and dry mass） were analyzed respectively. The results showed that soil water content， soil pH and soil organic carbon content increased significantly（P<0.05） under the combination of shading stress and exogenous NO， while the contents of total nitrogen， alkali-hydrolyzable nitrogen， total phosphorus and available phosphorus in soil increased but not significantly， and the soil C/N and C/P values reached significant level， and the C/N value increased significantly（P<0.05）； Correlation analysis showed that there was a positive correlation between soil pH value and organic carbon content（P<0.05）， the change of soil organic carbon content was positively correlated with plant height and leaf fresh mass（P<0.05）. It indicated that external application of SNP could promote the increase of soil pH under light-shading stress， change soil organic carbon content， increase C/N value， improve soil fertility， and promote leaf growth and development， then increase the biomass of C. roseus， and provide basis for scientific balanced fertilization and alkaloid content accumulation of C. roseus.

Key words: Soil organic carbon, growth index, Catharanthus roseus, exogenous NO donor

CLC Number:

Q945.34

Ying LIU, Jiayi WU, Ling JIN, Qianru JI, Yujie FU, Dewen LI. Effects of Exogenous NO on Soil Nutrient Content and Seedling Growth Characteristics from Potted Catharanthus roseus under Light-shading Stress[J]. Bulletin of Botanical Research, 2022, 42(6): 1088-1095.

Figures/Tables 5

Fig.1

Table 1

Table 2

Table 3

Table 4

References 28

1	刘柿良，潘远智，杨容孑，等.外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase及矿质营养吸收的影响［J］.植物营养与肥料学报，2014，20（2）：445-458.
	LIU S L， PAN Y Z， YANG R J，et al.Effects of exogenous NO on mineral nutrition absorption，lipid peroxidation and ATPase of plasma membrane in Catharanthus roseus tissues under cadmium stress［J］.Journal of Plant Nutrition and Fertilizer，2014，20（2）：445-458.
2	高赛，刘佳，唐玉情，等.长春花不同花色品种呈色的关键理化性质分析［J］.经济林研究，2022，40（1）：214-227.
	GAO S， LIU J， TANG Y Q，et al.Analysis on the key physicochemical properties of the coloration of varietals of Catharanthus roseus ［J］.Non-wood Forest Research，2022，40（1）：214-227.
3	柴梦颖，周士锋，冯林剑，等.长春花引种栽培关键技术与园林应用［J］.河南农业，2017，3（9），47-48，50.
	CAI M Y， ZHOU S F， FENG L J，et al.The key techniques for introduction and cultivation of Catharanthus roseus ［J］.Henan Agriculture，2017，3（9），47-48，50.
4	刘茳，潘会堂，张启翔.不同基质对岩生报春盆花生长发育的影响［J］.浙江农业学报，2013，25（3）：509-514.
	LIU J， PAN H T， ZHANG Q X.Effects on different substrates on growth of pot-planted Primula saxatilis ［J］.Acta Agriculturae Zhejiangensis，2013，25（3）：509-514.
5	乔娟峰，熊黑钢，王小平，等.基于最优模型的荒地土壤有机质含量空间反演［J］.江苏农业学报，2018，34（1）：68-75.
	QIAO J F， XIONG H G， WANG X P，et al.Spatial inversion of soil organic matter content in wasteland based on optimal model［J］.Jiangsu Journal Agricultural Sciences，2018，34（1）：68-75.
6	PUGA-FREITAS R， BLOUIN M.A review of the effects of soil organisms on plant hormone signalling pathways［J］.Environmental and Experimental Botany，2015，114：104-116.
7	朱晓婷，王克勤，陈敏全，等.昆明松华坝水源区不同土地利用方式对土壤有机碳及活性有机碳组分的影响［J］.东北林业大学学报，2016，44（2）：26-30.
	ZHU X T， WANG K Q， CHEN M Q，et al.Effects of three different land use types on soil organic carbon and active organic carbon fractions in water source area of Songhuaba，Kunming［J］.Journal of Northeast Forestry University，2016，44（2）：26-30.
8	郭素娟，谢明明，张丽，等.板栗细根碳、氮、磷化学计量时间变异特征［J］.植物营养与肥料学报，2018，24（3）：825-832.
	GUO S J， XIE M M， ZHANG L，et al.Temporal variation of C，N，P stoichiometric in fine roots of Castanea mollissima ［J］.Journal of Plant Nutrition and Fertilizers，2018，24（3）：825-832.
9	李瑞波.从有机碳营养的视角透视农作物现象［J］.磷肥与复肥，2013，28（5）：4-7.
	LI R B.Perspective of phenomenon of crop from organic carbon nutrient［J］.Phosphate & Compound Fertilizer，2013，28（5）：4-7.
10	ZHU S D， LI R H， SONG J，et al.Different leaf cost-benefit strategies of ferns distributed in contrasting light habitats of sub-tropical forests ［J］.Annals of Botany，2015，117（3）：497-506.
11	LIU W D， SU J R.Effects of light acclimation on shoot morphology，structure，and biomass allocation of two Taxus species in southwestern China［J］.Scientific Reports，2017，6：353384.
12	LIU Y， ZHAO D M， ZU Y G，et al.Effects of low light on terpenoid indole alkaloid accumulation and related biosynthetic pathway genes expression in leaves of Catharanthus roseus seedlings［J］.Botanical Studies，2011，52（2）：191-196.
13	蔡卓山，师尚礼，谢森林，等.外源NO对水分胁迫下苜蓿种子萌发的影响［J］.核农学报，2013，27（11）：1777-1782.
	CAI Z S， SHI S L， XIE S L，et al.Effects of exogenous nitric oxide on germination of alfalfa seeds under water stress［J］.Journal of Nuclear Agricultural Sciences，2013，27（11）：1777-1782.
14	时振振，李胜，杨柯，等.盐胁迫下豌豆幼苗内外源NO的生理生化响应［J］.草业学报，2014，23（5）：193-200.
	SHI Z Z， LI S， YANG K，et al.Physiological and biochemical response of pea seedlings to endogenous and exogenous NO under salt stress［J］.Acta Prataculturae Sinica，2014，23（5）：193-200.
15	赵萌，魏小红.吸胀冷害下外源NO对紫花苜蓿种子萌发及抗氧化性的影响［J］.草业学报，2015，24（4）：87-94.
	ZHAO M， WEI X H.Effects of nitric oxide on Medicago sativa seed germination under imbibitional chilling［J］.Acta Prataculturae Sinica，2015，24（4）：87-94.
16	CORPAS F J， BARROSO J B， CARRERAS A，et al.Constitutive arginine-dependent nitric oxide synthase activity in different organs of pea seedlings during plant development［J］.Planta，2006，224（2）：246-254.
17	俞月凤，彭晚霞，宋同清，等.喀斯特峰丛洼地不同森林类型植物和土壤C、N、P化学计量特征［J］.应用生态学报，2014，25（4）：947-954.
	YU Y F， PENG W X， SONG T Q，et al.Stoichiometric characteristics of plant and soil C，N and P in different forest types in depressions between karst hills，southwest China［J］.Chinese Journal of Applied Ecology，2014，25（4）：947-954.
18	贺海升，王琼，裴忠雪，等.落叶松人工林球囊霉素相关土壤蛋白与土壤理化性质空间差异特性［J］.生态学杂志，2015，34（12）：3466-3473.
	HE H S， WANG Q， PEI Z X，et al.Spatial variations of glomalin-related soil protein in Larix gmelinii plantations and possible relations with soil physicochemical properties［J］.Chinese Journal of Ecology，2015，34（12）：3466-3473.
19	张川，陈洪松，张伟，等.喀斯特坡面表层土壤含水量、容重和饱和导水率的空间变异特征［J］.应用生态学报，2014，25（6）：1585-1591.
	ZHANG C， CHEN H S， ZHANG W，et al.Spatial variation characteristics of surface soil water content，bulk density and saturated hydraulic conductivity on Karst slopes［J］.Chinese Journal of Applied Ecology，2014，25（6）：1585-1591.
20	张丽芳，胡海林.土壤酸碱性对植物生长影响的研究进展［J］.贵州农业科学，2020，48（8）：40-43.
	ZHANG L F， HU H L.Research progress on effect of soil pH on plant growth［J］.Guizhou Agricultural Sciences，2020，48（8）：40-43.
21	许自成，王林，肖汉乾.湖南烟区土壤pH分布特点及其与土壤养分的关系［J］.中国生态农业学报，2008，16（4）：830-834.
	XU Z C， WANG L， XIAO H Q.pH distribution and relationship to soil nutrient in Hunan tobacco lands［J］.Chinese Journal of Eco-Agriculture，2008，16（4）：830-834.
22	ZHAO W， CAI Z C， XU Z H.Does ammonium-based N addition influence nitrification and acidification in humid subtropical soils of China？［J］.Plant and Soil，2007，297（1/2）：213-221.
23	王春燕，李建华，刘汉文，等.不同林龄橡胶林土壤各组分碳的垂直分布及其相关关系［J］.林业资源管理，2017（3）：109-113，127.
	WANG C Y， LI J H， LI H W，et al.The vertical distribution and correlation of soil carbon in each component of rubber trees at different ages［J］.Forest Resources Management，2017（3）：109-113，127.
24	田冬，高明，徐畅.土壤水分和氮添加对3种质地紫色土氮矿化及土壤pH的影响［J］.水土保持学报，2016，30（1）：255-261.
	TIAN D， GAO M， XU C.Effects of soil moisture and nitrogen addition on nitrogen mineralization and soil pH in purple soil of three different textures［J］.Journal of Soil and Water Conservation，2016，30（1）：255-261.
25	黎嘉成，高明，田冬，等.秸秆及生物炭还田对土壤有机碳及其活性组分的影响［J］.草业学报，2018，27（5）：39-50.
	LI J C， GAO M， TIAN D，et al.Effects of straw and biochar on soil organic carbon and its active components［J］.Acta Prataculturae Sinica，2018，27（5）：39-50.
26	汪红霞.有机肥施用对土壤有机质变化及其组分影响的研究［D］.保定：河北农业大学，2014.
	WANG H X.Impact of organic fertilization on soil organic matter and composition［D］.Baoding：Agriculture University of Hebei，2014.
27	姚志霞，陈浩宁，周怀平，等.黄土旱塬24年不同秸秆还田土壤碳、氮、磷和胞外酶计量特征［J/OL］.环境科学.［2022-09-29］..
	YAO Z X， CHEN H N， ZHOU H P，et al.Effects of 24 years different straw return on soil carbon，nitrogen，phosphorus，and extracellular enzymatic stoichiometry in dryland of the loess plateau，China［J/OL］.Environmental Science.［2022-09-29］..
28	于晓梅，王非.海南长春花生境土壤环境及其生长规律［J］.东北林业大学学报，2010，38（6）：38-39，42.
	YU X M， WANG F.Suitable soil conditions for Catharanthus roseus in longlou of Hainan and its growth regularity［J］.Journal of Northeast Forestry University，2010，38（6）：38-39，42.

处理 Treatments	C/N	C/P	N/P
CK	86.36±4.29b	148.24±12.66b	1.71±0.09b
T1	72.16±7.89b	165.94±34.73a	2.42±0.731a
T2	81.19±8.67b	170.83±38.94a	2.08±0.33ab
T3	93.48±2.74a	152.40±16.11b	1.62±0.14b

处理 Treatments	指标 Factors	土壤有机碳 Soil organic carbon	土壤全磷 Soil total phosphorus	土壤全氮 Soil total nitrogen
CK	土壤pH Soil pH	0.66	-0.89	-1.00^**
CK	土壤含水量 Soil water content	-0.46	0.97	0.97
T1	土壤pH Soil pH	-0.75	-0.64	0.81
T1	土壤含水量 Soil water content	0.005	0.76	-0.05
T2	土壤pH Soil pH	0.38	0.27	-0.37
T2	土壤含水量 Soil water content	-0.28	-0.44	-0.35
T3	土壤pH Soil pH	0.86^*	0.03	0.46
T3	土壤含水量 Soil water content	0.71	0.38	0.61

处理 Treatments	鲜质量 Fresh mass /g	干质量 Dry mass /g	株高 Plant height /cm	叶长 Leaf length /cm	叶宽 Blade width /cm	叶面积 Leaf area /cm²	叶间距 Leaf spacing /cm	茎直径 Stem diameter /cm
CK	3.76±0.06b	0.62±0.02b	15.4±1.32b	6.56±0.16ab	2.40±0.19a	9.20±1.11a	0.92±0.03c	0.25±0.02c
T1	3.38±0.03b	0.55±0.02b	16.8±1.93ab	5.78±0.24b	2.32±0.11a	9.00±0.44a	1.18±0.06ab	0.34±0.02b
T2	2.96±0.05c	0.54±0.03b	17.40±1.28ab	6.06±0.42ab	2.34±0.14a	8.80±0.48a	1.08±0.03b	0.30±001bc
T3	5.30±0.24a	0.80±0.09a	21.00±1.22a	6.90±0.41a	2.50±0.08a	9.50±0.63a	1.30±0.06a	0.52±0.02a

处理 Treatments	指标 Factors	株高 Plant height	叶长 Leaf length	叶宽 Leaves wide	叶面积 Leaf area	鲜质量 Fresh mass	干质量 Dry mass
CK	土壤有机碳 Soil organic carbon	0.61	-0.50	0.500	0.92	-0.50	-0.50
	土壤全氮 Soil total nitrogen	-0.99	1.00^**	-1.000^**	-0.13	1.00^**	-0.50
	土壤全磷 Soil total phosphorus	-0.90	0.95	-0.950	0.19	0.95	-0.75
T1	土壤有机碳 Soil organic carbon	-0.11	0.20	0.007	0.93^**	0.34	0.12
	土壤全氮 Soil total nitrogen	0.08	-0.37	-0.200	-0.94^**	-0.52	-0.47
	土壤全磷 Soil total phosphorus	-0.47	0.13	-0.380	0.60	0.20	-0.05
T2	土壤有机碳 Soil organic carbon	0.07	0.45	0.740^*	0.32	0.30	-0.18
	土壤全氮 Soil total nitrogen	-0.22	0.10	-0.710	0.38	0.36	0.55
	土壤全磷 Soil total phosphorus	-0.40	0.41	-0.370	0.49	0.49	0.37
T3	土壤有机碳 Soil organic carbon	0.83^*	0.66	0.780^*	0.62	0.74^*	0.67
	土壤全氮 Soil total nitrogen	0.19	0.65	0.250	0.63	0.64	0.54
	土壤全磷 Soil total phosphorus	-0.04	0.43	0.120	0.32	0.38	0.27

[1]	Xiao-Ju ZHAO, Yi-Ting ZHANG, Jia LIU, Yang LIU, Zhong-Hua TANG. Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress [J]. Bulletin of Botanical Research, 2021, 41(4): 633-640.
[2]	JIA Shan-Shan, WANG Yan-Yan, YU Fang. Ultrasound-Assisted Enhancement of Hair Induction Efficiency and Transgenic Hairy Root Construction of Catharanthus roseus [J]. Bulletin of Botanical Research, 2018, 38(4): 604-610.
[3]	WANG Xi-Gang, ZHAN Ya-Guang, ZHANG Gui-Qin, YAN Chao-Fu, LI Xiang, ZENG Fan-Suo. Influences of Fertilization on Growth and Fruiting of Fraxinus mandschurica [J]. Bulletin of Botanical Research, 2017, 37(2): 298-303.
[4]	DING Yi-Yang1；MAO Zi-Jun1；ZHANG Ling2；DING Li3. Comparison of Soil Organic Carbon Stock of Betula costata* Secondary Forest and Mixed Broadleaved Korean Pine Original Forest in Xiaoxing’an Mountains. [J]. Bulletin of Botanical Research, 2015, 35(4): 604-611.
[5]	LIU Jian-Cai;CHEN Jin-Ling;JIN Guang-Ze*. Response of Soil Organic Carbon and Nutrients to Simulated Nitrogen Deposition in Typical Mixed Broadleaved-Korean Pine Forest [J]. Bulletin of Botanical Research, 2014, 34(1): 121-130.
[6]	SU Dong-Xue;WANG Wen-Jie*;QIU Ling;WANG Hong-Yan;AN Jing;ZHENG Guang-Yu. Correlation between Spectrophotometric Absorbance of Soil Solution and Contents of Variable Carbon and Nitrogen in Larch Plantation Forests [J]. Bulletin of Botanical Research, 2012, 32(3): 348-353.