Responses of Supplemental UV-B Radiation to Physiological Properties and Secondary Metabolites of Thlaspi arvense

doi:10.7525/j.issn.1673-5102.2022.06.018

Abstract

Abstract:

In order to understand the growth rule of the medicinal plant Thlaspi arvense under the enhanced ultraviolet（UV-B） radiation， the 2-months-old seedlings of T. arvense were taken as materials， two different radiation intensities were supplemented artificially with 3.26 μW·cm^-2（T1） and 9.78 μW·cm^-2（T2） on the basis of natural light， and the T. arvense grown under natural light as the control， to investigate the response of various physiological indicators and secondary metabolites to different UV-B radiation intensities， such as photosynthetic indicators and chlorophyll fluorescence parameters， photosynthetic pigments（chlorophyll a and b） content， osmotic adjustment substances（malondialdehyde， soluble protein， soluble sugar and proline） content， activity of antioxidant enzymes（SOD， POD， CAT and APX）， and the content of total flavonoids， total phenols and sinigrin. The results showed： net photosynthetic rate， stomatal conductance， transpiration rate， intercellular CO₂ concentration， maximum fluorescence（F_m）， PSⅡ maximum photochemical efficiency（F_v/F_m） and Potential Photochemical Efficiency of PSⅡ at Two Radiation Intensities（F_v/F₀） and other chlorophyll fluorescence parameters decreased with the increasing of radiation intensity respectively. However， the chlorophyll a and chlorophyll b， osmotic adjustment substances， such as soluble protein， soluble sugar and proline， superoxide dismutase（SOD）， peroxidase（POD）， catalase（CAT） and ascorbate catalase（APX）， total phenols and sinigrin firstly increased and then decreased with the increase of radiation time， while the total flavonoids content gradually increased， which was significantly different（P＜0.05） from the control in the late radiation period. According to the comprehensive analysis of the research results， under different radiation intensities， T. arvense might improve the synthesis of total flavonoids and total phenols， the accumulation of soluble protein and soluble sugar， and the content of chlorophyll， and the activity of superoxide dismutase and peroxidase and catalase increased， to resist the influence of ultraviolet stress， and then showed the resistance and the adaptability to the stress environment of adversity. The results provided a theoretical basis for breeding and further utilization of T. arvense.

Key words: Thlaspi arvense, UV-B radiation, physiological characteristics, secondary metabolites

CLC Number:

S567.9

Zhihui HUANG, Yining ZHANG, Nana LI, Baojiang ZHENG, Yuhong ZHANG. Responses of Supplemental UV-B Radiation to Physiological Properties and Secondary Metabolites of Thlaspi arvense[J]. Bulletin of Botanical Research, 2022, 42(6): 1079-1087.

Figures/Tables 6

Table 1

Table 2

Fig.1

Fig.2

Fig.3

Fig.4

References 30

1	杨扬，焦宏彬，刘洋，等.UV-B辐射对神农香菊光合作用的影响［J］.东北林业大学学报，2017，45（1）：30-32.
	YANG Y， JIAO H B， LIU Y，et al.Effects of UV-B on photosynthesis in Dendranthema indicum var.aromaticum ［J］.Journal of Northeast Forestry University，2017，45（1）：30-32.
2	祁虹，段留生，王树林，等.全生育期UV-B辐射增强对棉花生长及光合作用的影响［J］.中国生态农业学报，2017，25（5）：708-719.
	QI H， DUAN L S， WANG S L，et al.Effect of enhanced UV-B radiation on cotton growth and photosynthesis［J］.Chinese Journal of Eco-Agriculture，2017，25（5）：708-719.
3	黄勇，周冀衡，郑明，等.UV-B对烟草生长发育及次生代谢的影响［J］.中国生态农业学报，2009，17（1）：140-144.
	HUANG Y， ZHOU J H， ZHENG M，et al.Effect of UV-B on growth and development and secondary metabolism of flue-cured tobacco［J］.Chinese Journal of Eco-Agriculture，2009，17（1）：140-144.
4	周新明.UV-B辐射增强对葡萄叶片光合生理及总酚含量的影响［D］.杨凌：西北农林科技大学，2007.
	ZHOU X M.The effects of enhanced Ultraviolet-B radiation on photosynthetic physiology and total hydroxybenzene in the leaves of grapevine［D］.Yangling：Northwest A & F University，2007.
5	刘英，于雪莹，于莉莉，等.UV-B辐射下不同树龄杜仲叶片光合及部分生理特性的变化［J］.东北林业大学学报，2020，48（6）：42-46，50.
	LIU Y， YU X Y， YU L L，et al.Changes of photosynthetic and physiological characteristics in Eucommia ulmoides oliver leaves of different tree ages under UV-B radiation［J］.Journal of Northeast Forestry University，2020，48（6）：42-46，50.
6	褚润，陈年来，韩国君，等.三种UV-B辐射强度下香蒲的生长和抗氧化状况［J］.湿地科学，2020，18（1）：32-39.
	CHU R， CHEN N L， HAN G J，et al.Growth and antioxidant status of Typha orientalis under 3 kinds of UV-B radiation intensities［J］.Wetland Science，2020，18（1）：32-39.
7	李锦馨，张阁，马燕，等.UV-B辐射胁迫对药用植物次生代谢产物的影响研究进展［J］.河南农业科学，2018，47（5）：1-7.
	LI J X， ZHANG G， MA Y，et al.Research progress on impacts of enhanced UV-B radiation on medicinal plants' secondary metabolites［J］.Journal of Henan Agricultural Sciences，2018，47（5）：1-7.
8	卢克欢，苏贝贝，郭双，等.UV-B辐射对颠茄氮代谢及次生代谢产物含量的影响［J］.中国生物化学与分子生物学报，2017，33（10）：1062-1069.
	LU K H， SU B B， GUO S，et al.Effects of UV-B on the activities of nitrogen metabolism and secondary metabolites of Atropa belladonna ［J］.Chinese Journal of Biochemistry and Molecular Biology，2017，33（10）：1062-1069.
9	中国科学院中国植物志编辑委员会.中国植物志：第33卷［M］.北京：科学出版社，1987：80．
	Editorial Committee of The Flora of China，Chinese Academy of Sciences.Flora of China：Volume 33［M］.Beijing：Science Press，1987：80.
10	国家药典委员会.中华人民共和国药典：一部［M］. 2020年版.北京：中国医药科技出版社，2020：321.
	Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China：Vol.1［M］.2020 Edition.Beijing：China Medical Science Press，2020：321.
11	ZHAO R， WEI M X， SHI G Y，et al.One-pot process for simultaneously obtaining oil and sinigrin from field pennycress（Thlaspi arvense） seeds using microwave-assisted biphasic extraction［J］.Industrial Crops and Products，2021，166：113483.
12	JIANG M Y， ZHANG J H.Effect of abscisic acid on active oxygen species，antioxidative defence system and oxidative damage in leaves of maize seedlings［J］.Plant and Cell Physiology，2001，42（11）：1265-1273.
13	高俊凤.植物生理学实验指导［M］.北京：高等教育出版社，2006.
	GAO J F.Experimental guidance for plant physiology［M］.Beijing：Higher Education Press，2006.
14	ZHANG J X， KIRKHAM M B.Antioxidant responses to drought in sunflower and Sorghum seedlings［J］.New Phytologist，1996，132（3）：361-373．
15	付立忠，赵利梅，刘骞，等.氮素形态对三叶青块根主要化学成分含量及抗氧化活性的影响［J］.中国药学杂志，2021，56（8）：633-639.
	FU L Z， ZHAO L M， LIU Q，et al.Effects of nitrogen forms on main phytochemical content and antioxidant activity in root tubers of Tetrastigma hemsleyanum ［J］.Chinese Pharmaceutical Journal，2021，56（8）：633-639.
16	陈玉，周旻，伍丽萍，等.HPLC测定菥蓂子中的黑芥子苷［J］.华西药学杂志，2012，27（1）：94-95.
	CHEN Y， ZHOU M， WU L P，et al.Determination of sinigrin in Thlaspi arvense by HPLC［J］.West China Pharmaceutical Journal of Pharmaceutical Sciences，2012，27（1）：94-95.
17	JORDAN B R.Rieview：molecular response of plant cells to UV-B stress［J］.Functional Plant Biology，2002，29（8）：909-916.
18	王海霞，刘文哲.UV-B辐射增强对喜树叶片色素含量和形态结构的影响［J］.中国农学通报，2011，27（5）：209-213.
	WANG H X， LIU W Z.Effects of enhanced UV-B radiation on leaf morphology and structure and pigment contents of Camptotheca acuminata ［J］.Chinese Agricultural Science Bulletin，2011，27（5）：209-213.
19	张雅梅，茹广欣，肖梦雨，等.干旱胁迫对泡桐幼苗生长和叶绿素荧光参数的影响［J］.中南林业科技大学学报，2021，41（6）：22-30.
	ZHANG Y M， RU G X， XIAO M Y，et al.Influence of drought stress on the growth and chlorophyll fluorescence of Paulownia seedling［J］.Journal of Central South University of Forestry & Technology，2021，41（6）：22-30.
20	张玉红，陈路瑶，刘彤，等.增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响［J］.中国农学通报，2018，34（4）：76-82.
	ZHANG Y H， CHEN L Y， LIU T，et al.The growth and photosynthetic physiological responses of Phellodendron amurense seedlings under supplementary UV-B radiation［J］.Chinese Agricultural Science Bulletin，2018，34（4）：76-82.
21	卢克欢，刘兴，杨怡，等.UV-B胁迫下Ca²⁺对颠茄生理特性与次生代谢产物的调控研究［J］.作物学报，2018，44（10）：1527-1538.
	LU K H， LIU X， YANG Y，et al.Effect of exogenous Ca²⁺ on physiological characteristics and secondary metabolites accumulation of Atropa belladonna L.seedlings under UV-B stress［J］.Acta Agronomica Sinica，2018，44（10）：1527-1538.
22	回嵘，李新荣，赵锐明，等.UV-B辐射对生物结皮层藓类植物生理生化指标的影响［J］.干旱区地理，2014，37（6）：1222-1230.
	HUI R， LI X R， ZHAO R M，et al.Effects of enhanced UV-B radiation on physiological and biochemical indexes of moss from biological soil crusts［J］.Arid Land Geography，2014，37（6）：1222-1230.
23	徐子棋，张瑜，杨献坤.吉林省西部菊芋品质及耐盐碱性优选研究［J］.森林工程，2019，35（6）：6-15.
	XU Z Q， ZHANG Y， YANG X K.Optimization research of quality and saline-alkaline resistance of Helianthus tuberosus L.species in the Western Jilin Province［J］.Forest Engineering，2019，35（6）：6-15.
24	孟凡来，郭华春.不同甘薯品种抗UV-B辐射增强的效应分析［J］.中国农业气象，2019，40（5）：293-300.
	MENG F L， GUO H C.Effect analysis of anti-UV-B enhancement of two sweet potato cultivars［J］.Chinese Journal of Agrometeorology，2019，40（5）：293-300.
25	杨璐，赵天宏.UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响［J］.华北农学报，2018，33（5）：174-180.
	YANG L， ZHAO T H.The effect of enhanced UV-B radiation on soybean root’s activate oxygen metabolism and antioxidative system［J］.Acta Agriculturae Boreali-Sinica，2018，33（5）：174-180.
26	刘学良，姚俊修，刘翠兰，等.7个接骨木无性系苗木对盐胁迫的生理响应与评价［J］.中南林业科技大学学报，2021，41（1）：37-44，79.
	LIU X L， YAO J X， LIU C L，et al.Physiological response and evaluation of 7 clones of Sambucus williamsii during seedling stage to salt damage stress［J］.Journal of Central South University of Forestry & Technology，2021，41（1）：37-44，79.
27	DWIVEDI R， SINGH V P， KUMAR J，et al.Differential physiological and biochemical responses of two Vigna species under enhanced UV-B radiation［J］.Journal of Radiation Research and Applied Sciences，2015，8（2）：173-181.
28	阎秀峰，王洋，李一蒙.植物次生代谢及其与环境的关系［J］.生态学报，2007，27（6）：2554-2562.
	YAN X F， WANG Y， LI Y M.Plant secondary metabolism and its response to environment［J］.Acta Ecologica Sinica，2007，27（6）：2554-2562.
29	孟祥才，王喜军.活性氧促进道地药材质量形成的假说及其探讨［J］.中草药，2011，42（4）：799-804．
	MENG X C， WANG X J.Postulate about active oxygen improving quality of genuine Chinese medicinal materials and its exploration［J］.Chinese Traditional and Herbal Drugs，2011，42（4）：799-804．
30	THOMAS T T D， PUTHUR J T.UV-B priming enhances specific secondary metabolites in Oryza sativa（L.） empowering to encounter diverse abiotic stresses［J］.Plant Growth Regulation，2020，92（2）：169-180.

荧光参数 Fluorescence parameters	处理 Treatment
荧光参数 Fluorescence parameters	CK	T1	T2
初始荧光 F_o	0.48±0.02a	0.51±0.05a	0.49±0.02a
最大荧光 F_m	2.41±0.04c	1.68±0.13b	1.42±0.06a
最大光合效率 F_v/F_m	0.80±0.01c	0.70±0.01b	0.65±0a
潜在光化学效率 F_v/F₀	4.06±0.25c	2.32±0.08b	1.87±0.02a
非光化学猝灭系数 Q_NP	0.45±0.03a	0.59±0.03b	0.62±0.01b
光化学猝灭系数 Q_p	0.80±0.01c	0.67±0.01b	0.54±0.04a
电子传递速率 R_ET	28.70±1.28c	23.57±1.24b	19.87±1.56a

[1]	Shuyao ZHUANG, Hengbo XU, Xiaoyu HU, Shang DAI, Yanni ZHANG. Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings [J]. Bulletin of Botanical Research, 2023, 43(4): 520-530.
[2]	Qing-Yan GAI, Zi-Ying WANG, Yu-Jie FU, Jiao JIAO, Hui-Mei WANG, Yao LU, Jing LIU, Jin-Xian FU, Xiao-Jie XU, Lan YAO. Effects of UV-B Radiation on Phenolic Acid Accumulation and Physiological and Biochemical Characters in 84K Poplar [J]. Bulletin of Botanical Research, 2021, 41(6): 878-887.
[3]	Sen SHI, Na MIAO, Yu-Tong SHI, Hui-Mei WANG. Effects of Sucrose on Growth and Physiological Characteristics in vitro Plantlets of 84K Poplar [J]. Bulletin of Botanical Research, 2021, 41(1): 74-78.
[4]	LI Dong-Lin, JIN Ya-Qin, CUI Meng-Fan, HUANG Lin-Xi, PEI Wen-Hui. Effects of Shading on Physiological Characteristics and Ultrastructure of Mesophyll Cell of Emmenopterys henryi Leaves [J]. Bulletin of Botanical Research, 2020, 40(1): 29-40.
[5]	JI Li, HAN Jiao, WANG Fang, WANG Jun, SONG Di, ZHANG Li-Jie, QI Yong-Hui, YANG Yu-Chun. Effects of Drought Stress on Photosynthetic and Physiological Characteristics of Juglans mandshurica Seedlings in Different Soil Substrates [J]. Bulletin of Botanical Research, 2019, 39(5): 722-732.
[6]	XIE Zhi-Yu, ZHANG Wen-Hui, WEI Yong-Tao. Morphological Variations and Physiological Characteristics of Phragmites australis in Three Habitats in Yellow River Delta [J]. Bulletin of Botanical Research, 2019, 39(3): 372-379.
[7]	FAN Yan-Min, DANG Shi-Kun, WANG Wen-Jie, WANG Hui-Mei. Effect of Carbon Source, Auxin and Elicitor on the Growth and Synthesis of Secondary Metabolites of Adventitious Roots of Cajanus cajan(Linn.) Millsp. [J]. Bulletin of Botanical Research, 2018, 38(3): 391-398.
[8]	LI Min;QU Dan;YU Hai-Di;WANG Hui-Mei. Characteristics and Biochemical Indexes of Cajanus cajan* Hairy Root [J]. Bulletin of Botanical Research, 2014, 34(3): 339-342.
[9]	MENG Qing-Huan;ZU Yuan-Gang;GUO Xiao-Rui;DUAN Xi-Hua;. Effect of Exogenous Nitric Oxide on Photosynthetic Pigments and Chlorophyll Fluorescence under Enhanced UV-B Radiation in Larix gmelinii* [J]. Bulletin of Botanical Research, 2013, 33(2): 181-185.
[10]	WEI Xiao-Li;ZHENG Na;LI Xiao-Yang;HAN Rong. Effect of Enhanced UV-B Radiation on Arabidopsis* Mesophyll Cell Protein [J]. Bulletin of Botanical Research, 2013, 33(2): 186-190.
[11]	ZHAO Fa;ZHAO Xiao-Ying*;JIN Wan-Gui;DONG Zheng-Wu. Responses of Three Native Shrubs to Drought Stress in Urumqi [J]. Bulletin of Botanical Research, 2010, 30(6): 692-696.
[12]	AN Xu-Liang;HAN Rong*. Renovation of ROP GTPase in Young Seedlings of Wheats by He-Ne Laser under Enhanced UV-B Radiation [J]. Bulletin of Botanical Research, 2010, 30(6): 725-730.
[13]	GAO Zeng-Lu;GAO Yu-Bao;ZHENG Zhi-Rong;ZHANG Xiao-Chun. A Comparative Study of Photosynthetic Characteristics of Caragana davazamcii* Between Plants of Different Ages in Huangfuchuan Basin of Ordos Plateau [J]. Bulletin of Botanical Research, 2009, 29(2): 182-186.
[14]	SUN Shou-Hui;LI Wei;LÜChang-Li;GAO Guo-Ping;Qi Jin-Yu;XU Zhe. Extraction and Analysis of Secondary Metabolites in Poplar Phloem [J]. Bulletin of Botanical Research, 2009, 29(1): 120-123.
[15]	ZHANG Yu-Dong;JIANG Zhong-Zhu. Physiological Responses of Iris to NaHCO₃ Stress [J]. Bulletin of Botanical Research, 2009, 29(1): 49-53.