Identification and Expression Pattern Analysis of TkAPC10 in Taraxacum kok-saghyz Rodin

doi:10.7525/j.issn.1673-5102.2022.05.014

Abstract

Abstract:

APC/C is an E3 ubiquitin-ligase complex that is involved in regulating cell-cycle progression. In order to reveal the function of the APC/C complex in Taraxacum kok-saghyz Rodin（TKS）， TkAPC10 was identified， and its expression pattern was analyzed to preliminarily determine its function in this study. The ORF of TkAPC10 was 579 bp， encoding 192 amino acids. Its genomic DNA sequence was 1 092 bp， contained 6 exons and 5 introns. Genome analysis showed that TkAPC10 was a single copy in TKS genome. In addition to the TATA-box and CAAT-box enhancer elements， TkAPC10 promoter contained cis-acting elements related to ABA， JA， light， and stress response. Phylogenetic analysis suggested that APC10 from different species showed high homology. The similarity between TKAPC10 and lettuce LsAPC10 was up to 99%， and the similarity to APC10 from other Compositae plants was also over 95%. The expression pattern of TKAPC10 was analyzed by qRT-PCR， and the results indicated that TKAPC10 expression in the tissues with vigorous cell division（such as flowers， leaves and roots） was significantly higher than that with relatively slower cell division （such as pedicel）. Under exogenous ABA treatment， the transcript level of TKAPC10 was obviously decreased， while it was significantly increased after MeJA and ET treatments. Under PEG6000 and mannitol treatments， the expression of TKAPC10 was obviously decreased， but its expression was markedly induced by salt stress treatment. The results in this study suggested that TKAPC10 was involved in the regulation of cell division， hormone signaling and abiotic stress response in TKS.

Key words: Taraxacum kok-saghyz Rodin, TkAPC10, cell division, hormone signal transduction, abiotic stress

CLC Number:

S576

Bi QIN, Xiaoxiao WANG, Yushuang YANG, Qiuhai NIE, Qiuhui CHEN, Shizhong LIU. Identification and Expression Pattern Analysis of TkAPC10 in Taraxacum kok-saghyz Rodin[J]. Bulletin of Botanical Research, 2022, 42(5): 830-839.

Figures/Tables 7

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引物名称 Primer name	引物序列 Primer sequence（5′—3′）
TkAPC10-F	ATGGCTACGGAGTCATCCGA
TkAPC10-R	TCATCTCACAGTTGAGTAAG
TkAPC10-QF	AGGGGGAAGAAGAAACGAAA
TkAPC10-QR	GAAGAGAAGAGACGCCATTG
TkActin-F	GGAAGGATCTTTATGGGAAC
TkActin-R	CAGACACTATACTTCCTCTCAGG

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