Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress

doi:10.7525/j.issn.1673-5102.2022.03.012

Abstract

Abstract:

Low temperature is a kind of abiotic stress in the growth process of Hemerocallis fulva. The transcriptome data of Hemerocallis fulva leaves under cold treatment groups （10， 5， 0 ℃） and control group （15℃） was compared， and a total of 2 457 DEGs were screened， including 1 253 up-regulated genes and 1 204 down-regulated genes. The results showed that differentially expressed genes（DEGs） were mainly concentrated in 49 GO processes such as cellular process， metabolic process and catalytic activity， and 42 KEGG metabolic pathways， including metabolic pathways， biosynthesis of secondary metabolites and plant hormone signal transduction. Among them， the DEGs involved in plant hormone signal transduction pathway changed in varying degrees， GH3.10 gene was increased to 13.624 times higher than the control group， IAA1 gene was down-regulated to 0.120 times； the DEGs involved in soluble sugar synthesis pathway also changed by 0.076-28.114 times. Then， 29 DEGs shared by treatment groups were analyzed by heat map and gene regulation networks. Based on the position of genes in gene regulation networks， the roles of ABCF5， OFPs and SWEETs in response to cold were analyzed respectively. The results of this study provide a theoretical basis for further exploring the key genes of low temperature response， germplasm development and molecular breeding of Hemerocallis fulva.

Key words: Hemerocallis fulva, low temperature stress, transcriptome, hormone, soluble sugar

CLC Number:

S682

Dongmei HUANG, Ying CHEN, Lu BAI, Di’an NI, Yiyang XU, Zhiguo ZHANG, Qiaoping QIN. Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress[J]. Bulletin of Botanical Research, 2022, 42(3): 424-436.

Figures/Tables 11

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基因 Gene ID	正向序列 Sense primes（5′—3′）	反向序列 Antisense primers（3′—5′）
内参UBQ	AACGTGAAGGCCAAGATAC	AGACGGAGCACCAGGTGGA
DN3322_c0_g1	TATCTCGTCCATCCTGTC	TCCATCGGTCTTTCTATT
DN56313_c2_g3	TAGCGATGAGGATAGAATA	CCCGGCTTTGGACTGGAA
DN61425_c1_g1	AACTGCGGCTCGGTTGAG	CCCACGGCTTTGATTTCG
DN60592_c1_g2	TTGTATCGGTTTAGGTGA	AACATTGGTTTACGCTTG
DN56941_c1_g4	TGAGGAGCAAAACCAAAA	CCTCCCCGAAGCCACAAC
DN62545_c4_g4	AAATACGTGGCAAATCGT	CAACACCCAAGAGCAAAA
DN60404_c3_g2	GGGGAATGTGATATGACTG	CGCAACTTGTAGGGAGAT
DN48576_c0_g1	GGAGGAAGCACAAGCAGC	CAGCAAGCAAGTCGGATG
DN59631_c1_g1	GGCAGAAACTGAAACTAA	CATTCGGACCATACAAGG
DN60001_c3_g1	CTGCATAAAGGCCATAGT	CTTCACCCAATACCAATC
DN487206_c0_g1	CTCACAACGGTTCATCCA	AAGAGGGAGTAGGGAATG
DN61874_c2_g2	GCTGACATCTCGTGGTTC	CAGCCTTGCCACAAATAA

Group	Raw_reads	Clean_reads	Clean_bases	Error /%	Q20 /%	Q30 /%	GC /%
T₀	46 929 604	46 376 031	6.44GB	0.02	98.24	94.57	45.47
T₁	42 571 478	42，120 296	5.86GB	0.02	98.18	94.45	45.17
T₂	41 846 630	41 392 792	5.74GB	0.02	98.29	94.68	45.35
T₃	44 823 304	44 378 448	6.16GB	0.02	98.30	94.73	45.88

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