Optimization on Extraction of Total Phenols by Response Surface from Saxifraga stolonifera and Its Correlation in Inhibiting the Activity of PLA2 in Deinagkistrodon acutus Venom

doi:10.7525/j.issn.1673-5102.2022.02.011

Abstract

Abstract:

To reveal the relationship between total phenols（TP） of Saxifraga stolonifera and inhibition of the activity of phospholipase A₂（PIR） in Deinagkistrodon acutus venom， we used the single factor and Box-Behnken methods to optimize the extraction conditions of TP of Saxifraga stolonifera and PIR. The effect of various experimental factors on the yield of TP and PIR in the extraction of S. stolonifera by ultrasonic assisted extraction were investigated respectively， and the correlation between the content of TP and PIR were analyzed. The results showed that when the particle size was 60 mesh， the material to liquid ratio was 1∶50， the ultrasonic power was 250 W， and the ultrasonic time was 1.8 h， the content of TP in the aqueous extract of S. stolonifera and its inhibitory rate（PIR） of the PLA₂ activity in D. acutus venom could reach the optimal ultrasonic-assisted extraction process conditions. The content of TP was （86.90±0.46） mg·g^-1， and the PIR was（40.91±0.21）%. The results of correlation analysis showed that the content of TP and the PIR were extremely significant correlation（P<0.01）， indicating that the TP might be the material basis of S. stolonifera to inhibit the PLA₂activity in D. acutus venom .

Key words: Saxifraga stolonifera, total phenols, PLA₂ activity, Deinagkistrodon acutus venom, response surface optimization

CLC Number:

Q949.751.2

Tongtong Sun, Zhongjuan Wang, Xiujuan Zhang, Shihan Yang, Xiaoqiang Chen, Ying Zhang. Optimization on Extraction of Total Phenols by Response Surface from Saxifraga stolonifera and Its Correlation in Inhibiting the Activity of PLA₂ in Deinagkistrodon acutus Venom[J]. Bulletin of Botanical Research, 2022, 42(2): 259-267.

Figures/Tables 5

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Table 1

Table 2

Fig.2

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References 22

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序号 No.	试验设计 The experiment design				试验结果 The experimental results
序号 No.	A /目	B （g：mL）	C /W	D /h	TP含量 /（mg·g^-1）	Std.Dev	PIR /%	Std.Dev
1	60	1∶40	200	1	69.5	1.05	30.9	0.317
2	40	1∶40	200	2	88.0	0.75	31.3	0.269
3	80	1∶40	300	1	66.5	1.11	29.6	0.250
4	40	1∶20	300	2	78.8	1.06	29.8	0.225
5	60	1∶60	300	1	80.2	0.87	48.8	0.291
6	80	1∶40	200	2	71.8	1.25	17.7	0.317
7	60	1∶20	300	1	67.2	0.96	26.0	0.309
8	60	1∶20	300	3	75.2	0.40	34.0	0.244
9	60	1∶40	300	2	79.0	1.18	40.7	0.290
10	60	1：40	300	2	80.4	0.78	44.7	0.313
11	60	1∶40	300	2	78.0	0.76	41.8	0.276
12	40	1∶60	300	2	90.3	0.75	33.3	0.121
13	60	1∶60	200	2	83.4	0.95	28.8	0.296
14	40	1∶40	300	1	82.9	1.15	42.8	0.267
15	60	1∶40	300	2	78.2	0.66	43.9	0.301
16	60	1∶60	300	3	91.1	0.90	25.1	0.273
17	80	1∶20	300	2	64.4	6.47	23.7	0.206
18	60	1∶40	200	3	84.4	1.00	24.2	0.266
19	40	1∶40	400	2	86.7	0.91	23.0	0.234
20	60	1∶60	400	2	84.2	0.75	28.6	0.221
21	60	1∶20	400	2	69.5	1.10	40.4	0.270
22	60	1∶40	300	2	79.0	0.64	42.8	0.307
23	40	1∶40	300	3	92.1	1.18	18.7	0.272
24	60	1∶40	400	1	74.2	1.37	38.9	0.295
25	80	1∶40	400	2	73.0	0.96	22.9	0.270
26	80	1∶60	300	2	76.5	0.95	28.3	0.203
27	60	1∶20	200	2	71.8	1.00	19.1	0.327
28	80	1∶40	300	3	74.2	0.36	30.1	0.293
29	60	1∶40	400	3	84.4	0.49	20.3	0.310

来源 Source	TP含量 TP content /（mg·g^-1）					PIR /%
来源 Source	平方和 Sum of squares	自由度 df	均方 Mean square	F	P	平方和 Sum of squares	自由度 df	均方 Mean square	F	P
模型Model	2 151.15	14	153.65	36.47	<0.000 1^**	2 056.61	14	146.90	12.88	<0.000 1^**
A	711.48	1	711.48	168.89	<0.000 1^**	58.96	1	58.96	5.17	0.039 3^*
B	517.45	1	517.45	122.83	<0.000 1^**	33.00	1	33.00	2.89	0.111 1
C	0.80	1	0.80	0.19	0.669 5	40.70	1	40.70	3.57	0.079 8
D	309.07	1	309.07	73.37	<0.000 1^**	347.76	1	347.76	30.49	<0.000 1^**
AB	0.09	1	0.09	0.02	0.885 9	0.30	1	0.30	0.03	0.873 0
AC	1.56	1	1.56	0.37	0.552 3	45.56	1	45.56	3.99	0.065 5
AD	0.56	1	0.56	0.13	0.720 3	151.29	1	151.29	13.26	0.002 7^*
BC	2.40	1	2.40	0.57	0.462 7	115.56	1	115.56	10.13	0.006 6^*
BD	2.10	1	2.10	0.50	0.491 5	251.22	1	251.22	22.02	0.000 3^*
CD	5.52	1	5.52	1.31	0.271 4	35.40	1	35.40	3.10	0.099 9
A²	184.68	1	184.68	43.84	<0.000 1^**	525.70	1	525.70	46.08	<0.000 1^**
B²	309.79	1	309.79	73.54	<0.000 1^**	141.16	1	141.16	12.37	0.003 4*
C²	223.76	1	223.76	53.12	<0.000 1^**	602.79	1	602.79	52.84	<0.000 1^**
D²	214.34	1	214.34	50.88	<0.000 1^**	115.92	1	115.92	10.16	0.006 6^*
残差Residual	58.98	14	4.21			159.71	14	11.41
失拟项Lack of fit	30.93	10	3.09	0.44	0.866 0	149.48	10	14.95	5.85	0.051 7
纯误差Pure error	28.05	4	7.01			10.23	4	2.56
总误差Total error	2 210.13	28				2 216.31	28

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Optimization on Extraction of Total Phenols by Response Surface from Saxifraga stolonifera and Its Correlation in Inhibiting the Activity of PLA₂ in Deinagkistrodon acutus Venom

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