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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 252-258.doi: 10.7525/j.issn.1673-5102.2022.02.010

• Phytochemistry • Previous Articles     Next Articles

Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim. by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography

Jiaben Zhou1, Caidan Rezeng2, Maoxian Re1, Cairang Limao1,3()   

  1. 1.College of Life Sciences,Qinghai Normal University,Xining 810008
    2.College of Pharmacy,Qinghai University for Nationalities,Xining 810007
    3.College of Nationalities,Qinghai Normal University,Xining 810008
  • Received:2021-03-18 Online:2022-03-20 Published:2022-02-22
  • Contact: Cairang Limao E-mail:1224863256@qq.com
  • About author:Zhou Jiaben(1996—),male,postgraduate,research direction for Medicinal Phytochemistry.
  • Supported by:
    National Natural Science Foundation of China(31660086);Key Laboratory of Medicinal Animal and Plant Resources in Qinghai-Tibet Plateau of Qinghai Province(2017-ZJ-Y13)

Abstract:

An efficient method was established for the separation and preparation of chlorogenic acid, pedaliin-6″-acetate and rosmarinic acid from Dracocephalum tanguticum Maxim.. The chlorogenic acid, pedaliin-6″-acetate and rosmarinic acid was enriched by semi-preparative high performance liquid chromatography(SP-HPLC) from ethyl acetate extract, and then separated and purification by high-speed counter-current chromatography(HSCCC) and preparative high performance liquid chromatography(Pre-HPLC). A total of 54 mg of chlorogenic acid, 150 mg of pedaliin-6″- acetate and 200 mg of rosmarnic acid were obtained from ethyl acetate extract with purities of 96.9%, 97.9% and 95.1% by HPLC analysis, respectively. Their structures were identified by nuclear magnetic resonance spectrum. The result show that this method is suitable for the isolation and purification of chlorogenic acid, pedaliin-6″-acetate and rosmarnic acid from D. tanguticum. It also avoids the disadvantages of traditional separation methods such as many operations, large reagent consumption and non-recyclability, provides a reference basis for the study of separating of active components and preparation reference substance from D.tanguticum.

Key words: high-speed counter-current chromatography, preparative high-performance liquid chromatography, Dracocephalumtanguticum Maxim., chlorogenic acid, Pedaliin-6″-acetate, rosmarnic acid

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