Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis

doi:10.7525/j.issn.1673-5102.2022.02.006

Abstract

Abstract:

To lay a foundation for studying the molecular regulation mechanism of LTP family gene and molecular genetic improvement of Populus davidiana × P. alba var. pyramidalis， the expression pattern of LTP family genes from P. davidiana × P. alba var. pyramidalis was analyzed.The protein properties， multiple sequence alignments and phylogenetic tree analysis of LTP family members were performed to show their structural features. The P. davidiana × P. alba var. pyramidalis seedlings treated with gravity， salt and PEG stress respectively were subjected to quantitive real time PCR analysis. The expression patterns of PdbLTP family genes were carried out respectively. Eight PdbLTP genes and two subfamily PdbGLTP genes were obtained， and their CDS lengths were ranged from 294-396 bp. LTP family were hydrophobic proteins and had eight cysteine conserved structures， without transmembrane region. qRT-PCR analysis showed that the expression patterns of different PdbLTP family genes were different under gravity processing. Expression levels of PdbLTP1， PdbLTP3， PdbLTP5 and PdbLTP7 genes were up-regulated in tension wood compared to those in the normal wood. The transcripts levels of PdbLTP1， PdbLTP2， PdbLTP3， PdbLTP5 genes were abundant in stem.Expression of PdbLTP family genes was induced by salt stress except for PdbLTP5. PdbLTP1， PdbLTP2， PdbLTP3 and PdbLTP5 were up-regulated under drought stress. Different members of PdbLTP gene family play important roles in regulation of xylem development and tolerance to abiotic stress in Populus davidiana × P. alba var. pyramidalis.

Key words: Populus davidiana×P. alba var. pyramidalis, LTP family, bioinformatics, expression pattern analysis

CLC Number:

S792.114

Qian Sun, Yuhang Wu, Yaxuan Zhang, Jingdan Cao, Jingjing Shi, Chao Wang. Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis[J]. Bulletin of Botanical Research, 2022, 42(2): 211-223.

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引物 Primer	序列 Sequence（5′—3′）	引物 Primer	序列 Sequence（5′—3′）
PdbLTP1 F	CTTGCTGCAGTGGGTTGAAAG	PdbLTP7 F	GCCCCAACTTCTGACGCTGC
PdbLTP1 R	CTGCTTCGATTCTTCACTTC	PdbLTP7 R	CACTGGGAGAAATAGTGTAAG
PdbLTP2 F	CTGACCCTTGCTGCAAAGGAG	PdbLTP8 F	CTCATGATGTTTCTTTTACTC
PdbLTP2 R	GAAACGAAGAATAAGATGGAAG	PdbLTP8 R	GATTTATTTGCTTGGAAGCTG
PdbLTP3 F	CCAGTTGTGATATTCTGCTAG	PdbGLTP1 F	GAGAAACTCGTGGAAGAATC
PdbLTP3 R	GTGCTTTCAACCCACTGCAGC	PdbGLTP1 R	CCGTACAATATCAGAACAGAG
PdbLTP4 F	GTCATACTGTGAAAGCTGAC	PdbGLTP2 F	CCCAACAATGGCAGTTTTGAG
PdbLTP4 R	GATCAAGGTTGTGAAAAATGC	PdbGLTP2 R	GAACGTTTTATTATCAGGCAG
PdbLTP5 F	GCAAGTCCGAGAGCCCTTC	PdbEF1-α F	TGGGTCGTGTTGAAACTGGTGT
PdbLTP5 R	GGAAGTCCAGCAGCAAGAGC	PdbEF1-α R	GGCAGGATCGTCCTTGGAGTTC
PdbLTP6 F	CAGGAATCATTTCCATGTTG	Pdbactin F	GCTGAGAGATTCCGTTGCCCTG
PdbLTP6 R	CACCCCGGCACAACATGGTG	Pdbactin R	GGCGGTGATCTCCTTGCTCATT

基因名称 Gene names	核苷酸数量 Nucleotide number	氨基酸数量 Amino acid number
PdbLTP1	357	118
PdbLTP2	390	129
PdbLTP3	357	118
PdbLTP4	396	131
PdbLTP5	294	97
PdbLTP6	348	115
PdbLTP7	327	108
PdbLTP8	327	108
PdbGLTP1	720	239
PdbGLTP2	483	160

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