Effects of PEG Osmotic Treatment on Thermal Dormancy Induction and Water Status of Fraxinus mandshurica Seeds

doi:10.7525/j.issn.1673-5102.2021.06.008

Abstract

Abstract:

Taking the seeds of Fraxinus mandshurica as the material to release the initial dormancy， after two batches of seeds of pre-water absorption and non-pre-water absorption， at 25℃， with different concentrations （0， 25%， 50%） of PEG treatment different time（3 days， 7 days， 3 days PEG+4 days H₂O environment） and germination under suitable conditions. The changes of seed water content and seed germination performance during different treatments were determined to explore the changes of water content of seeds treated with PEG at different concentrations and the relationship between the water content and seed germination ability. The results showed that： after PEG treatment， the seed water content of seeds was as follows： contrast>25% PEG>50% PEG， that was， the lower the PEG concentration， the higher the seed water content. Among them，after PEG treatment， the water content of seeds treated with pre-water decreased， while the water content of seeds without pre-water increased gradually. At the same PEG concentration for the same time， the germination rate of seeds treated with pre-water and non-pre-water was significantly different， that was， whether PEG treatment or pre-water treatment all affected the change law of seed water content and seed germination ability. The seed water was negatively correlated with the seed germination rate. PEG osmotic treatment attenuated the induction of thermal dormancy of F. mandshurica seeds， which was related to seed water status）

Key words: Fraxinus mandschurica, thermal dormancy, PEG6000, water content

CLC Number:

S792

Cheng-Cheng CUI, Ming-Yue LI, Peng ZHANG. Effects of PEG Osmotic Treatment on Thermal Dormancy Induction and Water Status of Fraxinus mandshurica Seeds[J]. Bulletin of Botanical Research, 2021, 41(6): 904-910.

Figures/Tables 3

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Table 1

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References 23

1	闫月，卢艳，崔程程，等.聚乙二醇引发处理对水曲柳种子在高温下萌发的影响［J］.东北林业大学学报，2020，48（6）：13-16.
	Yan Y，Lu Y，Cui C C，et al.Effect of PEG priming treatment on the germination of Fraxinus mandshurica seeds at high temperature［J］.Journal of Northeast Forestry University，2020，48（6）：13-16.
2	Hilhorst H W M，Finch-Savage W E，Buitink J，et al.Dormancy in plant seeds［M］.//Lubzens E，Cerda J，Clark M.Dormancy and Resistance in Harsh Environments.Berlin，Heidelberg：Berlin，2010：43-67.
3	凌世瑜，董愚得.水曲柳种子休眠生理的研究［J］.林业科学，1983，19（4）：349-359.
	Ling S Y，Dong Y D.Researches on the physiology of seed dormancy ofmanchurian ash ［J］.Scientia Silvae Sinicae，1983，19（4）：349-359.
4	张鹏，孙红阳，沈海龙.温度对经层积处理解除休眠的水曲柳种子萌发的影响［J］.植物生理学，2007，43（1）：21-24.
	Zhang P，Sun H Y，Shen H L.Effect of temperature on germination of stratified seeds of Fraxinus mandshurica Rupr.［J］.Plant Physiology Communications，2007，43（1）：21-24.
5	李明月，赵彤彤，张鹏.聚乙二醇渗透处理对水曲柳种子在不同温度下萌发的影响［J］.东北林业大学学报，2019，47（6）：8-11.
	Li M Y，Zhao T T，Zhang P.Germination response of Fraxinus mandshurica seed to polyethylene glycol osmotic treatment under different temperatures［J］.Journal of Northeast Forestry University，2019，47（6）：8-11.
6	Hoang H H，Sotta B，Gendreau E，et al.Water content：a key factor of the induction of secondary dormancy in barley grains as related to ABA metabolism［J］.Physiologia Plantarum，2013，148（2）：284-296.
7	卢艳，闫月，崔程程，等.初生休眠解除状态和干燥处理对水曲柳种子萌发的影响［J］.植物研究，2020，40（4）：490-495.
	Lu Y，Yan Y，Cui C C，et al.Effects of primary dormancy-released status and drying treatment on germination of Fraxinus mandshurica seeds［J］.Bulletin of Botanical Research，2020，40（4）：490-495.
8	李瑛.不同小麦品种对干旱胁迫的生理生化响应［D］.兰州：兰州大学，2016.
	Li Y.Physiological and biochemical responses to drought stress different wheat cultivars［D］.Lanzhou：Lanzhou University，2016.
9	何玮，蒋安，王琳，等.PEG干旱胁迫对红三叶抗性生理生化指标的影响研究［J］.中国农学通报，2013，29（5）：5-10.
	He W，Jiang A，Wang L，et al.Study on the effects of PEG stress on physiological and biochemical indexes of five red clovers［J］.Chinese Agricultural Science Bulletin，2013，29（5）：5-10.
10	万燕，欧阳建勇，袁航，等.干旱胁迫对苦荞生理特征和红外光谱表征特性的影响［J］.成都大学学报：自然科学版，2020，39（3）：230-233，240.
	Wan Y，Ouyang J Y，Yuan H，et al.Effects of drought stress on physiological characteristics and infrared spectrum characterization of tartary buckwheat［J］.Journal of Chengdu University：Natural Science Edition，2020，39（3）：230-233，240.
11	Khan A A，Tao K L，Knypl J S，et al.Osmotic conditioning of seeds：physiological and biochemical changes［J］.Acta Horticulturae，1978，83（83）：267-278.
12	Cantliffe D J，Fischer J M，Nell T A.Mechanism of seed priming in circumventing thermodormancy in lettuce［J］.Plant Physiology，1984，75（2）：290-294.
13	Guedes A C，Cantliffe D J，Shuler K D，et al.Overcoming thermos dormancy in lettuce by seed priming［J］.Proceedings of the Florida State Horticultural Society，1979，92：130-133.
14	刘彦文，李明，姚东伟.引发对莴苣种子萌发及减轻其高温休眠的影响［J］.种子，2010，29（10）：54-56.
	Liu Y W，Li M，Yao D W.Effects of priming treatment on germination and decreasing dormancy at high temperature of lettuce seed［J］.Seed，2010，29（10）：54-56.
15	赵兴俊.向日葵种子休眠与水分含量的关系及休眠的破除［J］.中国农业信息，2015（21）：44-45.
	Zhao X J.Relationship between seed dormancy and water content and dormancy breaking of Helianthus annuus seeds［J］.China Agricultural Information，2015（21）：44-45.
16	张俊峰.关于向日葵种子休眠与水分含量的关系及休眠的破除研究［J］.农村科学实验，2018（1）：65-65.
	Zhang J F.Study on the relationship between seed dormancy and water content and dormancy breaking of Helianthus annuus seeds［J］.Experiment of Rural Science，2018（1）：65-65.
17	陈姗姗.山芹菜种子休眠特性及萌发生理的研究［D］.长春：吉林农业大学，2007.
	Chen S S.Studies on dormancy characteristics and germination physiology of Sanicula chinensis（kom.）kitag.seeds［D］.Changchun：Jilin Agricultural University，2007.
18	杨思根，史广富，施顺宝，等.人工干燥水稻种子二次休眠现象及对发芽率的影响［J］.上海农业科技，2008（6）：47-48.
	Yang S G，Shi G F，Shi S B，et al.Secondary dormancy of artificially dried Oryza sativa seeds and its effect on germination rate［J］.Shanghai Agricultural Science and Technology，2008（6）：47-48.
19	江城，李雪琴，史广富.人工干燥水稻种子二次休眠及不同破除方法对发芽率的影响［J］.现代农业科技，2009（17）：24.
	Jiang C，Li X Q，Shi G F.Effects of secondary dormancy and different breaking methods on germination rate of artificially dried Oryza sativa seeds［J］.Modern Agricultural Science and Technology，2009（17）：24.
20	赵笃乐，张树榛，郎韵芳.冬小麦种子的发育及其田间活力［J］.种子，1998，（6）：7-11.
	Zhao D L，Zhang S Z，Lang Y F.Development and field vigor of winter wheat seed［J］.Seed，1998（6）：7-11.
21	王景升，王凤英，刘玉香.不同成熟度玉米，高粱种子种用品质的研究［J］.种子，1982（2）：15-18.
	Wang J S，Wang F Y，Liu Y X.Study on the quality of Zea mays and Sorghum bicolor seeds with different maturity［J］.Seed，1982（2）：15-18.
22	Rasyad A，Van Sanford D A，Tekrony D M.Changes in seed viability and vigour during wheat seed maturation［J］.Seed Science and Technology，1990，18（2）：259-267.
23	赵笃乐，王志敏，吴中波.种子水分对小麦种子萌发与休眠调控的研究［J］.种子，2001（6）：22-23.
	Zhao D L，Wang Z M，Wu Z B.Study on effects of water content of seed on wheat seed dormancy and germination［J］.Seed，2001（6）：22-23.

预吸水与否 Pre-water or not	渗透处理时间 Permeation treatment time（d）	PEG浓度 PEG concentration（%）	发芽率 Germination rate（%）	发芽指数 Germination index	发芽时间 Germination time（d）
是Yes	3	0	44.00±4.32bc	0.74±0.05a	15.32±0.51ab
		25	84.00±5.89d	1.48±0.12c	14.50±0.20ab
		50	78.00±10.13d	1.23±0.14bc	15.94±0.41abc
	3（+4 H₂O）	0	16.00±3.65a	0.51±0.20a	13.20±1.82a
		25	41.00±6.61bc	0.58±0.11a	19.78±0.77c
		50	60.00±6.32c	0.88±0.11ab	19.55±1.33c
	7	0	30.00±10.39ab	0.60±0.27a	19.71±2.64c
		25	83.00±1.00d	1.20±0.03bc	17.76±0.47bc
		50	92.00±2.31d	1.30±0.05bc	18.32±0.30bc
否No	3	0	84.00±4.32de	1.36±0.08cd	16.08±0.65ab
		25	92.00±1.63ef	1.55±0.02d	15.15±0.15a
		50	96.00±1.63f	1.42±0.05d	17.25±0.45ab
	3（+4 H₂O）	0	31.00±11.47ab	0.58±0.20a	18.93±1.59b
		25	47.00±7.90bc	0.84±0.07ab	17.62±1.55ab
		50	67.00±3.42cd	0.98±0.04bc	18.32±0.09ab
	7	0	20.00±2.31a	0.49±0.25a	19.58±2.39b
		25	81.00±4.43de	1.21±0.06bcd	17.25±0.38ab
		50	81.00±9.00de	1.28±0.15cd	16.18±0.26ab

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