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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 824-835.doi: 10.7525/j.issn.1673-5102.2021.05.021

• Research report • Previous Articles    

Optimization on the Main Active Components Extraction Using Ultrasonic-assisted Method from Safflower

Yu LI1, Xiao YANG1, Xiao YE1, Jie-Qiong DENG1, Wei-Nian HUANG1, Sha DAI2()   

  1. 1.Industrial Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610300
    2.PanZhiHua Academy of Agricultural and Forestry Sciences,Panzhihua 617000
  • Received:2020-08-11 Online:2021-09-20 Published:2021-07-05
  • Contact: Sha DAI E-mail:daisweet@foxmail.com
  • About author:LI Yu(1987—),female,assistant researcher,mainly engaged in research on genetic breeding of Chinese medicinal materials.
  • Supported by:
    National Modern Agricultural Industry Technology System of China(CARS-21);Sichuan Province Special Projects in Crop and Livestock Breeding during the 13th Five-year Plan(2016NYZ0036-4-1);Sichuan Province Financial Innovation Ability Promotion Project(2016GYSH003);Sichuan Science and Technology Support Program(2014SZ0134)

Abstract:

To extract the main components from Carthamus tinctorius L., the Box-Behnken design method and response surface methodology were used to optimize the extraction process of C. tinctorius L.; Hydroxy-safflower yellow A and kaempferol-3-O-rutinoside were the main flavonoids in C. tinctorius L. petals . The results showed that Ultrasonic extraction method were the optimal extraction technology: using 57% methanol as solvent, liquid-material ratio was 0.1∶25 g·mL-1, ultrasonic temperature was 70℃, extraction time was 45 min, ultrasound power was 177 W, Under these conditions, hydroxy-safflower yellow A extraction yield was 1.74%, kaempferol-3-O-rutinoside extraction yield was 1.53%. The safflower main ingredient extraction was recommended to use ultrasonic extraction.

Key words: Carthamus tinctorius L., Hydroxy-safflower yellow A, kaempferol-3-O-rutinoside, extraction technology, response surface methodology

CLC Number: