Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 744-752.doi: 10.7525/j.issn.1673-5102.2021.05.013
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Xin-Tong JI1,2, Lei YU1,2, Ya-Guang ZHAN1,2()
Received:
2020-10-08
Online:
2021-09-20
Published:
2021-07-05
Contact:
Ya-Guang ZHAN
E-mail:yaguangzhan@126.com
About author:
JI Xin-Tong(1996—),female,postgraduate,mainly engaged in tree genetics and breeding reseach.
Supported by:
CLC Number:
Xin-Tong JI, Lei YU, Ya-Guang ZHAN. Cloning and Expression Analysis of BRASSINAZOLE RESISTANTANT1 (BZR1) Gene from Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2021, 41(5): 744-752.
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URL: http://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.05.013
Table 1
Primers sequences for cloning FmBZR1 gene and fluorescent quantification
引物名称 Primer name | 引物序列(5′-3′) Primer sequence | 用途 Usages |
---|---|---|
FmBZR1-F | 5′-ATGTGGGAAGCTGGAGGATCA-3′ | PCR扩增引物 Primers for PCR amplification primer |
FmBZR1-R | 5′-TTACATCCGGGTGTTTCCACTT-3′ | |
qFmBZR1-F | 5′-ATGCAGGGTTAAGGGCACAAGG-3′ | 荧光定量PCR引物 Primers for qRT-PCR |
qFmBZR1-R | 5′-ACCATCAGGCTCCACGATCCAA-3′ | |
Tu-F | 5′-AGGACGCTGCCAACAACTTT-3′ | 荧光定量PCR内参基因引物 Primers for qRT-PCR internal reference gene |
Tu-R | 5′-TTGAGGGGAAGGGTAAATAGTG-3′ |
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