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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 744-752.doi: 10.7525/j.issn.1673-5102.2021.05.013

• Research report • Previous Articles    

Cloning and Expression Analysis of BRASSINAZOLE RESISTANTANT1 (BZR1) Gene from Fraxinus mandshurica

Xin-Tong JI1,2, Lei YU1,2, Ya-Guang ZHAN1,2()   

  1. 1.School of Life Science,Northeast Forestry University,Harbin Heilongjiang 150040
    2.Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2020-10-08 Online:2021-09-20 Published:2021-07-05
  • Contact: Ya-Guang ZHAN E-mail:yaguangzhan@126.com
  • About author:JI Xin-Tong(1996—),female,postgraduate,mainly engaged in tree genetics and breeding reseach.
  • Supported by:
    Heilongjiang Province Applied Technology Research and Development Program(GA19B201)

Abstract:

To explore the expression characteristics of BZR1 gene under abiotic stress and hormone induction of brassinosteroid(BR) signaling on the growth and development of Fraxinus mandshurica and response to environmental stress,the target gene was cloned from F. mandshurica by PCR and its molecular structures were analyzed by bioinformatics. The expression patterns of FmBZR1 under low temperature, salt stress and ABA, IAA and GA3 hormones were investigated by real-time fluorescence quantitative PCR. Bioinformatics analysis showed that the FmBZR1 gene was 984bp, encoding 327 amino acids. FmBZR1 was a hydrophilic protein, and had high homology with BZR1 protein of Olea europaea var. sylvestris. The results of abiotic stress and hormone induction showed that the expression of FmBZR1 gene was significantly different from that of control group. The highest expression level of FmBZR1 gene was at 6 h after low temperature treatment and 24 h after salt treatment, which were 1.98 times and 10.13 times higher than that of the control group. After stress with ABA, IAA and GA3 for 3 h, the expression of FmBZR1 gene was the lowest, which was 0.52 times, 0.41 times and 0.50 times of the control group; after treatment GA3 for 24 h, the expression level of FmBZR1 gene was the highest, which was 6.23 times of the control group. FmBZR1 gene played a key role in the development and stress process of F. mandshurica.

Key words: Fraxinus mandshurica, FmBZR1, gene cloning, stress treatment, expression analysis

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