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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 738-743.doi: 10.7525/j.issn.1673-5102.2021.05.012

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Relationship Between Parental Genetic Distance and Heterosis of Growth Traitsin Pinus elliottii × P.caribaea var. Hondurensis Based on SNP marker

Yi-Liang LI1,2, Fen-Cheng ZHAO1,2, Yang LIU1,2, Sui-Ying ZHONG3, Chang-Ming LIN3, Zhi-Qiang TAN3, Fu-Ming LI3, Fang-Yan LIAO1,2, Hui-Shan WU1,2, Wen-Bin GUO1,2, Zhe WANG1,2   

  1. 1.Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520
    2.Guangdong Academy of Forestry,Guangzhou 510520
    3.Taishan Hongling Seed Orchard,Taishan 529200
  • Received:2020-05-21 Online:2021-09-20 Published:2021-07-05
  • About author:LI Yi-Liang(1979—),male,doctor,forest tree genetics and breeding.
  • Supported by:
    the Guangdong Forestry Science and Technology Innovation Program(2019KJCX019)


Using SNP loci to estimate the genetic distance between the parents of Pinus elliottii × P. caribaea, and effectively predicting the heterosis of tree height, diameter at breast height(DBH) and timber volume in their hybrids, which provides a reference for molecular marker-assisted mating design and breeding. Using SLAF-seq technology to sequence and analyze the DNAs of 131 germplasm resources from the parents of P. elliottii × P. caribaea, and valid SNP markers were obtained. Based on these SNP loci, the genetic distances between 13 parents of P. elliottii × P. caribaea were estimated by using MEGA 5.0 software, and cluster analysis were performed. The heterosis of the growth traits and the correlation between heterosis and SNP genetic distance of the 13 cross combinations were analyzed and calculated by using SPSS software. A total of 53 952 polymorphic SLAF tags and 96 736 valid SNP markers were obtained by SLAP-seq. The genetic distance between the parents of P. elliottii × P. caribaea was arranged from 0.425 1 - 0.490 6, and the correlation coefficients between SNP genetic distance of the parents and heterosis of their hybrids in tree height, DBH and timber volume were 0.680, 0.648 and 0.624, respectively. All three correlation coefficients reached a significantly positive correlation level. Utilizing SLAF-seq technology can obtain massive SNP loci. Based on these SNPs, the genetic distance between parents of P. elliottii × P. caribaea can be estimated, and the heterosis of tree height, DBH and timber volume in the hybrids can be effectively predicted.

Key words: Pinus elliottii × P.caribaea, SNP marker, genetic distance, heterosis

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