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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 729-737.doi: 10.7525/j.issn.1673-5102.2021.05.011

• Research report • Previous Articles    

Cloning and Promotor Analysis of DfMYB3 from Dendrocalamus farinosus

Tian-Tian DU1, Hui-Ping LI1, Bo-Ya WANG1, Qian OU1, Yan HUANG1, Ying CAO1, Shang-Lian HU1,2()   

  1. 1.Lab of Plant Cell Engineering Southwest University of Science and Technology,Mianyang 621010
    2.Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province,Mianyang 621010
  • Received:2020-12-29 Online:2021-09-20 Published:2021-07-05
  • Contact: Shang-Lian HU E-mail:hushanglian@126.com;hashanglian@126.com
  • About author:DU Tian-Tian(1995—),female,master,focused on biochemistry and molecular biology.
  • Supported by:
    Applied Basic Research Project of Sichuan Province(18YYJC0920);13th Five-Year Key Project of Sichuan Province(2016NYZ0038);Key Research and Development Projects of Sichuan Province(2019YFN0005);Environmentally friendly Energy Materials of State Key Laboratory Project(19fksy0113)

Abstract:

Based on the transcriptome database of Dendrocalamus farinosus, a MYB transcription factor named DfMYB3 was cloned from the leave of D. farinosus. Its open reading frame length was 1 287 bp, encoding 428 amino acids, and GenBank accession Number was KY963358. The conserved domain analysis showed that DfMYB3 had the typical SANT domains and DNA-Binding domains. Phylogenetic tree analysis showed that DfMYB3 clustered with R2R3-MYB transcription factors in SaccharumArabidopsis and Populus. Subcellular localization showed that DfMYB3 protein was expressed in both the nucleus and cell membrane,and was more significant in the nucleus. The sequence of 5′ flanking of DfMYB3 gene about 2 000 bp was cloned by chromosome walking method. Analysis of PlantCARE online software showed that the sequence had typical promoter characteristics, and contained hormones responsive elements such as GA, ABA, MeJA as well as stress responsive elements such as drought. The expression level of DfMYB3 gene was significantly up-regulated after 100 μmol·L-1 GA and 100 μmol·L-1 ABA treatment, indicating that DfMYB3 gene was involved in response to GA and ABA treatments. In order to explore the function of DfMYB3 promoter, the expression vector of GUS gene fused with DfMYB3 promoter was constructed and genetically transformed into tobacco. The results showed that GUS signal was detected in both leaf and stem of transgenic tobacco, especially in the veins.

Key words: Dendrocalamus farinosus, MYB transcription factor, promotor, GA and ABA treatments, GUS staining

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