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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 721-728.doi: 10.7525/j.issn.1673-5102.2021.05.010

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In vitro Propagation of Stem Axillary Buds of Three Species of Taxus Using Tissue Culture

Jiao JIAO, Jing LIU, Yu-Jie FU, Xin WANG, Qing-Yan GAI(), Yao LU, Xiao-Jie XU, Zi-Ying WANG   

  1. College of Chemistry,Chemical Engineering and Resource Utilization,Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:2021-03-11 Online:2021-09-20 Published:2021-07-05
  • Contact: Qing-Yan GAI E-mail:gqy_nefu@163.com
  • About author:JIAO Jiao(1986—),male,associate professor,majoring in plant cell, tissue, and organ culture technology.
  • Supported by:
    National Key R&D Program of China(2017YFD0600205);National Forestry and Grassland Science and Technology Innovation Program for Top Talented Youths(2020132611)


Taxus species are imminent extinction due to objective factors such as slow growth speed, decreased breeding ability, and weak competition, as well as subjective factors including overexploitation and habitat destruction. Therefore, the protection of Taxu resources via breeding methods has become the focus of research. In this study, three Taxus species in China(Taxus cuspidataT. mairei, and T. media) were selected as materials, and the stem tissues with axillary buds were selected as explants, and the different phytohormone combinations on the initiation, proliferation and rooting of axillary buds were systematically investigated. MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA was finally determined as the optimal medium for axillary bud initiation, and MS+0.4 mg·L-1 6-BA+1.0 mg·L-1 NAA was the suitable medium for adventitious bud proliferation. It was found that all three Taxus species could effectively initiated and proliferate in the above-mentioned medium. Moreover, WPM+0.8 mg·L-1 NAA+0.2 mg·L-1 IBA medium was found to promote the rooting of adventitious buds of T. cuspidata, and the WPM medium without adding any plant hormones was beneficial for the subsequent growth of rooted seedlings. Overall, this study established a simple and effective method for in vitro propagation of Taxus species, which would provide the technical support for the protection and sustainable utilization of Taxus resources in China.

Key words: Taxus species, stem axillary bud, propagation, in vitro culture

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