Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC

doi:10.7525/j.issn.1673-5102.2021.03.014

Abstract

Abstract:

In order to establish an HPLC method for the simultaneous determination of baschnaloside and rossicaside B in Boschniakia rossica dry whole， a chromatographic column： Klimail 100-5 C₁₈ column（250 mm×4.6 mm， 5 μm）； mobile phase： acetonitrile as mobile phase A， 0.5% formic acid aqueous solution as mobile phase B， gradient elution conditions： 0?12 min， 15% A； 12?30 min， 15%?20% A； 30?40 min， 20%?25% A； 40?45 min， 25%?30% A； 45?60 min， 30%?100% A； flow rate： 1.0 mL·min^-1； detection wavelength： 260 nm. Column temperature： 30℃. Injection volume： 20 μL. The linear range of naringin of baschnaloside was 4.688?150 μg·g^-1（R²=0.999 5）； the linear range of rossicaside B was 3.438?110 μg·g^-1（R²=0.999 1）； the average recoveries were 97.86% and 96.55%， and RSDs were 1.01 and 1.23（n=9）， respectively. A high-performance liquid chromatography method was developed for the simultaneous determination of two components， baschnaloside and rossicaside B in B.rossica. The methodological verification results show that the method is simple， fast， and reproducible， and can be used to determine the contents of baschnaloside and rossicaside B in B.rossica.

Key words: Boschniakia rossica, HPLC, baschnaloside, rossicaside B

CLC Number:

S567.9

Min CHEN, Meng-Fei TIAN, Chang-Yin FENG, Xian-Ming MENG, Meng LUO, Fan-Song MU. Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC[J]. Bulletin of Botanical Research, 2021, 41(3): 436-440.

Figures/Tables 6

Table 1

Fig.1

Table 2

Table 3

Repeatability and stability test results of each component

测定成分

Determination of compounds

重复性Repeatability

稳定性Stability

保留时间 $±$ SD

Retention time $±$ SD

RSD

(%)

平均峰面积 $±$ SD

Average peak area

RSD

(%)

草苁蓉纳拉苷

Baschnaloside

17.193

±

0.057

0.501

9 161.39

±

9.956

0.29

草苁蓉苷B

Rossicaside B

40.077

±

0.021

0.531

3 340.73

±

5.915

0.75

Table 3

Table 4

Table 5

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测定成分 Determination of compounds	样品量 Sample size(μg)	加入量 Sample size(μg)	测得量 Sample size(μg)	回收率 Recovery(%)	平均回收率 Recovery(%)	RSD（%）
草苁蓉纳拉苷 Baschnaloside	101.01	81.54	177.55	97.26	97.86	1.01
	100.39	81.54	180.93	99.45
	101.04	81.54	176.58	96.71
	100.88	102.39	200.27	98.52
	100.54	102.39	196.93	97.04
	101.10	102.39	197.49	97.05
	100.82	127.07	221.89	97.37
	100.46	127.07	223.53	98.24
	100.05	127.07	225.07	99.10

测定成分 Determination of compounds	样品量 Sample size(μg)	加入量 Sample size(μg)	测得量 Sample size(μg)	回收率 Recovery(%)	平均回收率 Recovery(%)	RSD（%）
草苁蓉苷B Rossicaside B	201.04	161.27	353.31	97.52	96.55	1.23
	200.20	161.27	348.47	96.40
	201.21	161.27	349.48	96.41
	200.19	202.80	397.99	98.76
	200.59	202.80	382.99	94.94
	201.18	202.80	391.99	97.03
	200.77	290.13	470.90	95.93
	200.06	290.13	475.19	96.94
	200.15	290.13	466.28	95.10

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