Genetic Diversity Assessment of Rhododendron sinofalconeri with Genotyping by Sequencing(GBS)

doi:10.7525/j.issn.1673-5102.2021.03.013

Abstract

Abstract:

Rhododendron sinofalconeri has high ornamental value with its pure yellow flower and bell-shaped corollas clustered on the top of the branches. In recent years， due to the anthropogenic impact of road construction， illegal excavation and other human activities， its habitat was severely fragmented and its population was shrunk. Therefore， it was urgent to research on conservation biology of R.sinofalconeri. Single-nucleotide-polymorphism（SNP） site mining and genetic diversity analysis were performed on two surviving populations of Laojunshan（LJS） and Daheishan（DHS） using genotyping-by-sequencing（GBS） technology. As a consequence， analysis of the 103 133 high-quality SNPs revealed that the two populations had lower genetic diversity at the species level（N_e=1.308 6， H_o=0.187 8， and H_e=0.185 6）， and higher genetic differentiation（F_st=0.176 5） at population level. The value of gene flow（N_m） between populations was 1.167 4. By cluster analysis， principal component analysis and population genetic structure analysis， 36 individuals were clustered into two different genetic groups. This study revealed the current situation of relatively low genetic diversity of R.sinofalconeri for the first time， which was helpful to further understand the endangered and scientific formulation of conservation measures.

Key words: Rhododendron sinofalconeri, GBS, SNP, Genetic structure, Reintroduction

CLC Number:

S685.21

Xu ZHANG, Xiu-Jiao ZHANG, Yong-Peng MA, Zheng-Hong LI, You-Ming WAN, Hong MA. Genetic Diversity Assessment of Rhododendron sinofalconeri with Genotyping by Sequencing(GBS)[J]. Bulletin of Botanical Research, 2021, 41(3): 429-435.

Figures/Tables 7

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来源 Origins	等位基因数 N_a	有效等位基因数 N_e	观测杂合度 H_o	期望杂合度 H_e	多态信息含量 PIC	核苷酸多样性 P_i
A	1.539 0	1.310 5	0.189 4	0.185 2	0.149 4	0.206 5
B	1.548 6	1.311 2	0.188 5	0.186 4	0.150 7	0.207 9
C	1.454 3	1.291 0	0.194 8	0.170 9	0.136 8	0.205 1
A1	1.682 5	1.319 8	0.185 2	0.196 1	0.160 4	0.205 7
B1	1.583 9	1.309 6	0.187 1	0.187 7	0.152 6	0.205 2
C1	1.581 1	1.309 6	0.182 1	0.187 6	0.152 4	0.205 1
平均Average	1.564 9	1.308 6	0.187 8	0.185 6	0.150 4	0.205 9

种群Population	A	B	C	A1	B1	C1
A	—	0.001 700	0.012 700	0.194 800	0.194 100	0.192 000
B	0.001 650	—	-0.000 200	0.189 600	0.189 300	0.187 500
C	0.012 650	-0.000 230	—	0.199 900	0.201 100	0.199 100
A1	0.177 000	0.172 700	0.181 200	—	-0.002 000	0.004 900
B1	0.176 400	0.172 500	0.182 200	-0.002 000	—	0.001 900
C1	0.174 700	0.171 000	0.180 500	0.004 882	0.001 932	—

种群Population	A	B	C	A1	B1	C1
A	—
B	151.265 2	—
C	19.512 8	-1 071.830 0	—
A1	1.162 4	1.197 6	1.129 7	—
B1	1.167 2	1.199 3	1.122 1	-125.188 0	—
C1	1.181 0	1.212 0	1.135 0	50.958 5	129.149 6	—

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