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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 743-750.doi: 10.7525/j.issn.1673-5102.2020.05.013

• Research Report • Previous Articles    

Expression of MYB Genes of Birch in Response to Hormones,Salt and Drought

LIU Jia-Xin, LIU Hui-Zi, SHI Jing-Jing, YU Ying, WANG Chao   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2020-03-05 Published:2020-07-10
  • Supported by:
    Science Foundation of China(31470671)

Abstract: Transcription factors are involved in different signaling pathways of plant hormones and the regulation of abiotic stress. In order to study the response of birch MYB transcription factor family genes to different hormone signals and abiotic stress, we used wild-type birch(Betula platyphylla) seeds:10 μmol·L-1 methyl jasmonate(MeJA), 100 μmol·L-1 ethylene glycol(ETH), 20 μmol·L-1 abscisic acid(ABA), 50 μmol·L-1 cytokinin(KT), 200 mmol·L-1 NaCl, 100 mmol·L-1 Mannitol and water were used as controls. Phenotypic observation and gene expression analysis were performed on hormone-treated 4 weaks birch hypocotyls. The hormone treatment could affect the growth of birch hypocotyls and radicles. The hypocotyls and roots of the treated birch were shorter than those of the water-treated control, and the KT-treated birch hypocotyls and roots were longer than those of the water-treated control; MeJA, Ethylene, and ABA treatment negatively regulated the expression of most BpMYBs, while BpMYB2,8,12 genes were up-regulated. After KT treatment, only BpMYB11 was down-regulated, and other BpMYBs genes were up-regulated. After NaCl treatment, most genes showed an expression pattern of down-regulation and then up-regulation, and was highly induced at 48 h; Mannitol simulated under drought conditions, most of the gene expression pattern showed slight increase after the first cut. These results indicate that birch MYB family genes can respond to hormone, salt and drought treatments, and play a role in regulating the development of birch hypocotyls and radicles in response to external signals. This study provides data and materials for exploring the molecular mechanism of hormone-regulated woody plant growth and development.

Key words: Betula platyphylla, BpMYB genes, stress treatment, expression pattern

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