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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 686-695.doi: 10.7525/j.issn.1673-5102.2020.05.007

• Research Report • Previous Articles    

Chloroplast Genome Characterization and Identification of Genetic Relationship of Relict Endangered Plant Amygdalus nana

YANG Bin, MENG Qing-Yao, ZHANG Kai, DUAN Yi-Zhong   

  1. College of Life Science, Yulin University, Yulin 719000
  • Received:2020-04-08 Published:2020-07-10
  • Supported by:
    National Natural Science Foundation of China(41601059)

Abstract: The chloroplast genome of Amygdalus nana in the Tertiary Period endangered plant was analyzed for structural characteristics, and its phylogenetic relationship with related species was explored. Getting the chloroplast genome through the Illumina HiSeq Xten for which to assemble, annotate and make feature analysis. The results show:①The chloroplast genome of A.nana is a circular tetrad structure with a total length of 158 596 bp, of which the length of LSC is 86 771 bp, the length of SSC is 19 037 bp, and both IRs are 26 394 bp. A total of 130 genes were annotated in the chloroplast genome of A.nana, including 85 PCGs, 37 tRNA and 8 rRNA. ②The expansion and contraction of IR boundary regions of 6 plants were analyzed, and there were some differences between the gene types and distribution in 4 boundary regions, and the closer the relationship was, the smaller the difference was. ③The 71 SSRs loci were predicted in the chloroplast genome of A.nana. ④By phylogenetic analysis, in the Subgen. Amygdalus, A.nana is closer to A.mongolica in phylogenetic relationship, but slightly farther from A.triloba and A.pedunculata. A large number of chloroplast genomes of angiosperms were involved, and the chloroplast genomes of A.nana were deeply analyzed to provide reference data for the evolutionary relationship and plant identification of Amygdalus L.

Key words: Amygdalus nana, chloroplast genome, genetic relationship, High-throughput sequencing

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