Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (4): 514-522.doi: 10.7525/j.issn.1673-5102.2020.04.005

• Research Report • Previous Articles    

Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’

DU Yi-Ming1, ZHONG Yuan1, SHANG Hong-Qin2, CHENG Fang-Yun1   

  1. 1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Peony International Institute, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083;
    2. College of Agricultural and Biological Engineering, Heze University, Heze 274015
  • Received:2019-12-15 Published:2020-06-12
  • Supported by:
    Research of Efficient Propagation Technology of Ornamental Plants in Beijing(2019-KJC-02-10-1);World-Class Discipline Construction and Characteristic Development Guidance Funds for Beijing Forestry University(2019XKJS0324)

Abstract: With the filaments of Paeonia ostii as explants, the effect of developmental stage of explant, basal media and PGRs on callus induction, proliferation and differentiation were investigated, and the morph-histological observation of different types of callus was performed. The translucent and white filaments from flower bud of 13-19 mm in diameter is optimal for callus induction. The optimal callus induction medium was SH containing 1 mg·L-1 2,4-D, 2 mg·L-1 NAA and 0.2 mg·L-1 BA. The optimal proliferation medium was SH containing 0.5 mg·L-1 2,4-D, 0.5 mg·L-1 NAA and 0.25 mg·L-1 BA, and the proliferation rate was 3.86. The filaments callus can be divided into three types according to their morphological and microstructural characteristic, the type Ⅰ callus presented typical embryogenic callus characteristics, and somatic embryogenesis was observed from this kind of callus; there were plenty of vascular tissues formed in the type Ⅱ and type Ⅲ callus, and shoot organogenesis was observed from the type Ⅱ callus, but no morphogenesis from the type Ⅲ callus. These results suggested that the filaments have important regeneration potential as a good novel explant source, which have important value in establishing in vitro regeneration system of tree peony.

Key words: Paeonia ostii ‘Feng Dan’, filament, callus

CLC Number: