Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO3

doi:10.7525/j.issn.1673-5102.2020.03.011

Abstract

Abstract: We studied the reversal effect of AgNO₃ on the hyperhydric Scutellaria baicalensis plantlets, and the contents of the active components. AgNO₃ had a significant recovery effect on biological activities of the restored hyperhydric S.baicalensis plantlets and the recovery effect was affected by the concentration of AgNO₃. When the concentration of AgNO₃ was 4 mg·L^-1, the recovery rate of hyperhydric plantlets was 88%, and the recovered S.baicalensis plantlets had the best growth state. We studied the contents of active components and biological activities of hyperhydric S.baicalensis plantlets recovered by AgNO₃ for one month. The content of baicalin in recovered hyperhydric S.baicalensis plantlets was 68.6 mg·g^-1, significantly higher than that in hyperhydric plantlets(38.3 mg·g^-1), but lower than that in normal tissue cultured plantlets(108.2 mg·g^-1). The results of antioxidant, antimicrobial and anti-tumor experiments showed that the recovered hyperhydric S.baicalensis plantlets were significantly better than the hyperhydric plantlets, but still lower than the normal tissue-cultured plantlets. Therefore, AgNO₃ had a good recovery effect on the hyperhydric plantlets of S.baicalensis.

Key words: hyperhydricity, AgNO₃, baicalin, biological activities

CLC Number:

Q949.777.6

WANG Sheng-Fang, HAN Shu-Lan, DANG Shi-Kun, WANG Hui-Mei. Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO₃[J]. Bulletin of Botanical Research, 2020, 40(3): 401-406.

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Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO₃

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