UPLC-Q-TOF-MS Analysis of Flavonoids in Flowers and Leaves of Camellia chuangtsoensis

doi:10.7525/j.issn.1673-5102.2019.03.006

Abstract

Abstract: The components and contents of flavonoids in flowers including petals and stamens and leaves including old leaves and new leaves of Camellia chuangtsoensis were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry. The results showed that fourteen flavonoids were detected in C.chuangtsoensis. The flavonoids in cluding luteolin, luteolin-7-O-rutinoside, quercetin-3, 7-O-diglucoside, narirutin, eriodictyol and genistin were identified in section Chrysantha Chang of Camellia for the first time, and quercetin-3, 7-O-diglucoside, narirutin, eriodictyol and genistinwere mainly found in the flowers. The contents of luteolin and luteolin-7-O-rutinosidein flowers were higher than thosein leaves, and the content of them in stamens were higher thanthose in petals. Quercetin-3-O-glucoside, quercetin-7-O-glucoside, quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were identified in leaves of section Chrysantha of Camellia for the first time, and the contents of them in leaves were far lower than that in flowers. The contents of the four flavonoids in old leaves were far lower than that in new leaves, and the contents of them in stamens were far lower than that in petals. The contents of catechin and epicatechin in flowers were higher than those in leaves, and the content of them in stamens were higher than that in petals. The contents of quercetin and kaempferol in flowers and leaves were very low. The flavonoids with higher contents in petals and stamens of C.chuangtsoensis were catechins, luteolins and quercetins, which mainly included catechin, luteolin and quercetin-3-O-glucoside. The flavonoids with higher contents in leaves were catechins and luteolins, which mainly includedcatechin, luteolin and luteolin-7-O-rutinoside. The contents of catechins, luteolins and total flavonoids in petals and stamens were higher than that in leaves, and the content of them in stamens were higher than that in petals. The contents of quercetins in petals and stamens were far higher than that in leaves, and the contents of them in petals were higher than that in stamens.

Key words: Camellia chuangtsoensis, flowers, leaves, flavonoids, ultraperformanceliquid chromatography quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)

CLC Number:

S685.14

LI Xin-Lei, WANG Jia-Tong, SUN Zhen-Yuan, WANG Jie, YIN Heng-Fu, FAN Zheng-Qi, LI Ji-Yuan. UPLC-Q-TOF-MS Analysis of Flavonoids in Flowers and Leaves of Camellia chuangtsoensis[J]. Bulletin of Botanical Research, 2019, 39(3): 365-371.

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