Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene

doi:10.7525/j.issn.1673-5102.2018.02.014

Abstract

Abstract: ThDUF106 gene were involved in the stress tolerance of Tamarix hispida, and the expression of ThDUF106 was up-regulated in T.hispida under salt-alkali stress. In order to study the function of ThDUF106 gene in T.hispida, the temporal and spatial expression of these gene was detected by qRT-PCR in the leaves and roots of three-month T.hispida under abiotic stress such as salinity, drought and heavy metals. The gene encoding this gene was also analyzed, and the gene was constructed and transformed into tobacco by the plant expression vector. Transgenic tobacco lines and wild-type one-month seedlings were treated, and physiological and biochemical indexes were compared and analyzed. The antioxidant status of transgenic and wild type tobacco was basically the same under various duress, indicating that the gene was not expressed in tobacco and could not improve the antioxidation of tobacco.

Key words: Tamarix hispida, ThDUF106, genetic transformation, tobacco, abiotic stress

CLC Number:

S793.5

GE Xiao-Lan, LIU Cai-Xia, ZHANG Xin-Xin, LI Ying, QU Guan-Zheng. Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene[J]. Bulletin of Botanical Research, 2018, 38(2): 260-267.

References

1. Qin F,Shinozaki K,Yamaguchi-Shinozaki K.Achievements and challenges in understanding plant abiotic stress responses and tolerance[J].Plant and Cell Physiology,2011,52(9):1569-1582.
2. Boyer J S.Plant productivity and environment[J].Science,1982,218(4571):443-448.
3. Endler A,Kesten C,Schneider R,et al.A mechanism for sustained cellulose synthesis during salt stress[J].Cell,2015,162(6):1353-1364.
4. Boudsocq M,Barbier-Brygoo H,Laurière C.Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana[J].The Journal of Biological Chemistry,2004,279(40):41758-41766.
5. Parida A K,Das A B.Salt tolerance and salinity effects on plants:a review[J].Ecotoxicology and Environmental Safety,2005,60(3):324-349.
6. Li Y,Liu P P,Takano T,et al.A chloroplast-localized rubredoxin family protein gene from Puccinellia tenuiflora(PutRUB) increases NaCl and NaHCO₃ tolerance by decreasing H₂O₂ accumulation[J].International Journal of Molecular Sciences,2016,17(6):804.
7. 张国军.盐胁迫对4种景天幼苗水势、荧光效率、丙二醛的影响[J].农业科技与装备,2012(7):3-6. Zhang G J.Effect of salt stress on the water potential,fluorescence efficiency,malonaldehyde of four stonecrop seedlings[J].Agricultural Science & Technology and Equipment,2012(7):3-6.
8. Liu J,Guo W Q,Shi D C.Seed germination,seedling survival,and physiological response of sunflowers under saline and alkaline conditions[J].Photosynthetica,2010,48(2):278-286.
9. 刘铎,丛日春,党宏忠,等.柳树幼苗渗透调节物质对中、碱性钠盐响应的差异性[J].生态环境学报,2014,23(9):1531-1535. Liu D,Cong R C,Dang H Z,et al.Comparative effects of salt and alkali stresses on plant physiology of willow[J].Ecology and Environmental Sciences,2014,23(9):1531-1535.
10. Zhu J K.Salt and drought stress signal transduction in plants[J].Annual Review of Plant Biology,2002,53:247-273.
11. Zhu J H,Lee B H,Dellinger M,et al.A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis[J].The Plant Journal,2010,63(1):128-140.
12. Guo L Q,Shi D C,Wang D L.The key physiological response to alkali stress by the alkali-resistant halophyte Puccinellia tenuiflora is the accumulation of large quantities of organic acids and into the rhyzosphere[J].Journal of Agronomy and Crop Science,2010,196(2):123-135.
13. Rosegrant M W,Cline S A.Global food security:challenges and policies[J].Science,2003,302(5652):1917-1919.
14. Guo L Q,Shi D C,Wang D L.The key physiological response to alkali stress by the alkali-resistant halophyte Puccinellia tenuiflora is the accumulation of large quantities of organic acids and into the rhyzosphere.J.Agron.Crop Sci,2010,196:123-135.
15. 邹原东,范继红.有机肥施用对土壤肥力影响的研究进展[J].中国农学通报,2013,29(3):12-16. Zou Y D,Fan J H.Review on effect of organic fertilizer on soil fertility[J].Chinese Agricultural Science Bulletin,2013,29(3):12-16.
16. Mishra A,Jha B.Antioxidant response of the microalga Dunaliella salina under salt stress[J].Botanica Marina,2011,54(2):195-199.
17. Sairam R K,Rao K V,Srivastava G C.Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress,antioxidant activity and osmolyte concentration[J].Plant Science,2002,163(5):1037-1046.
18. Jaoudé R A,De Dato G,De Angelis P.Photosynthetic and wood anatomical responses of Tamarix africana Poiret to water level reduction after short-term fresh-and saline-water flooding[J].Ecological Research,2012,27(5):857-866.
19. Gries D,Zeng F,Foetzki A,et al.Growth and water relations of Tamarix ramosissima and Populus euphratica on Taklamakan desert dunes in relation to depth to a permanent water table[J].Plant,Cell & Environment,2003,26(5):725-736.
20. Goodacre N F,Gerloff D L,Uetz P.Protein domains of unknown function are essential in bacteria[M].Bio,2013,5(1):e00744-13.
21. Cleverly J R,Smith S D,Sala A,et al.Invasive capacity of Tamarix ramosissima in a Mojave desert floodplain:the role of drought[J].Oecologia,1997,111(1):12-18.
22. De Baets S,Poesen J,Reubens B,et al.Root tensile strength and root distribution of typical Mediterranean plant species and their contribution to soil shear strength[J].Plant and Soil,2008,305(1-2):207-226.
23. Ma Q L,Wang J H,Li X R,et al.Long-term changes of Tamarix vegetation in the oasis-desert ecotone and its driving factors:implication for dryland management[J].Environmental Earth Sciences,2009,59(4):765-774.
24. Grisafi F,Oddo E,Gargano M L,et al.Tamarix arborea var.arborea and Tamarix parviflora:Two species valued for their adaptability to stress conditions[J].Acta Biol Hung,2016,67(1):42-52.
25. Farghaly F A,Radi A A,Abdel-Wahab D A,et al.Effect of salinity and sodicity stresses on physiological response and productivity in Helianthus annuus[J].Acta Biologica Hungarica,2016,67(2):184-194.
26. Kadukova J,Manousaki E,Kalogerakis N.Pb and Cd accumulation and phyto-excretion by salt cedar(Tamarix smyrnensis Bunge)[J].International Journal of Phytoremediation,2008,10(1):31-46.
27. Moreno-Jiménez E,Vázquez S,Carpena-Ruiz R O,et al.Using Mediterranean shrubs for the phytoremediation of a soil impacted by pyritic wastes in Southern Spain:a field experiment[J].Journal of Environmental Management,2011,92(6):1584-1590.
28. Luo C,Guo C,Wang W,et al.Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice[J].Plant Cell Rep,2014,33(2):323-336.

[1]	Luhua ZHOU, Junyi FANG, Zimo XIONG, Weifeng WU, Jiarui LIU, Qiao LU, Hongqing LING, Danyu KONG. Evaluation on Waterlogging Tolerance of Different Tomato Germplasm [J]. Bulletin of Botanical Research, 2023, 43(5): 657-666.
[2]	Xu HE, Yuan GAO, Qunye ZHANG, Chenguang ZHOU, Wei LI, Shuang LI. Establishment and Application of Genetic Transformation System for Populus simonii×P. nigra ‘Baicheng’ [J]. Bulletin of Botanical Research, 2023, 43(5): 667-678.
[3]	Chaoran SHAN, Xiaohu CHEN, Yunfei DING, Wei ZHAO, Han LU, Shangzhu GAO, Fenghui QI, Yaguang ZHAN, Fansuo ZENG. Functional Analysis of FmCCoAOMT Gene in Fraxinus mandshurica During Lignin Synthesis and Abiotic Stress [J]. Bulletin of Botanical Research, 2023, 43(5): 768-778.
[4]	Yu SUN, Yiteng ZHANG, Huihui CHENG. The Function of Salt and Alkaline Tolerance of WRKY42 Gene in Amorpha fruticosa [J]. Bulletin of Botanical Research, 2023, 43(4): 612-621.
[5]	Danni LI, Jiali LIU, Jitao ZHANG, Baoxiang GU, Fengjin ZHU, Qingjie GUAN. Cloning of WRKY40 Gene from Leymus chinensis and Analysis of Disease Resistance in Transgenic Tobacco [J]. Bulletin of Botanical Research, 2023, 43(3): 412-420.
[6]	Jinxia DU, Tingting SHEN, Haoran WANG, Yiping LIN, Huiyu LI, Lianfei ZHANG. Construction of Suppression Expression Vector and Genetic Transformation of BpSPL9 gene from Betula platyphylla [J]. Bulletin of Botanical Research, 2023, 43(1): 30-35.
[7]	Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Cloning and Functional Analysis of StNPR4 gene in Solanum tuberosum [J]. Bulletin of Botanical Research, 2022, 42(5): 821-829.
[8]	Bi QIN, Xiaoxiao WANG, Yushuang YANG, Qiuhai NIE, Qiuhui CHEN, Shizhong LIU. Identification and Expression Pattern Analysis of TkAPC10 in Taraxacum kok-saghyz Rodin [J]. Bulletin of Botanical Research, 2022, 42(5): 830-839.
[9]	Xueying WANG, Ruiqi WANG, Yang ZHANG, Cong LIU, Dean XIA, Zhigang WEI. Genome‑wide Identification and Stress Response Analysis of Cyclic Nucleotide-gated Channels(CNGC) Gene Family in Populus trichocarpa [J]. Bulletin of Botanical Research, 2022, 42(4): 613-625.
[10]	Bo-Chao ZHANG, Jia-Lin WANG, Yuan YIN, Yi-Da CHE, Jun-Jie DENG, Rong-Shu ZHANG. Tissue Expression Patterns of PdPapWRKY51 in Shanxin Poplar (Populus davidiana × P. alba var. pyramidlis) under Stress Conditions [J]. Bulletin of Botanical Research, 2021, 41(6): 911-920.
[11]	Xiao-Xiao WANG, Bi QIN, Yu-Shuang YANG, Qiu-Hai NIE, Ji-Chuan ZHANG, Shi-Zhong LIU. Cloning and Expression Analysis of E2 Ubiquitin-conjugating Enzyme Gene TkUBC2 in Taraxacum kok-saghyz Rodin [J]. Bulletin of Botanical Research, 2021, 41(1): 98-106.
[12]	JIANG Cheng, ZHANG Xi, TIAN Qing, LI Li. Isolation of the BpbHLH112 Gene and Expression Analysis of Its Promoter in Betula platyphylla [J]. Bulletin of Botanical Research, 2020, 40(4): 583-592.
[13]	XIAO Di, LIU Yi, LI Kai-Long, ZHENG Mi, QU Guan-Zheng. Genetic Transformation and Function Analysis of PsnHB13 Gene Isolated from Populu ssimonii×P.nigra in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2020, 40(4): 593-601.
[14]	MA Miao-Miao, LI Cheng-Hao, LIU Xiao, ZHANG Xin, YANG Jing-Li. Cloning and Bioinformatics Analysis of LoERF017 Gene from Larix kaempferi [J]. Bulletin of Botanical Research, 2020, 40(4): 602-612.
[15]	ZHANG Lei, XIONG Huan-Huan, CAO Qing, ZHAO Jia-Li, ZHANG Han-Guo. Drought Resistance of Larch NAC Gene by Transient Genetic Transformation [J]. Bulletin of Botanical Research, 2020, 40(3): 394-400.