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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (6): 870-875.doi: 10.7525/j.issn.1673-5102.2017.06.010

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Effects of Exogenous Nitric Oxide on Exogenous Gene Expression and DNA Methylation in Transgenic Birch

SUN Feng-Kun1, LI Si-Da1, LI Ji-Xiang1, CHEN Xiao-Hui1, ZENG Fan-Suo1,2   

  1. 1. College of Life science, Northeast Forestry University, Harbin 150040;
    2. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Nation, Northeast Forestry University, Harbin 150040
  • Received:2017-03-16 Online:2017-11-15 Published:2017-11-25
  • Supported by:
    The central university basic research business expenses special fund project(2572014DA04);National Natural Science Foundation of China(J1210053,31200463)

Abstract: In order to investigate the relationship between transgene expression and the methylation of genomic DNA induced by 2 mmol·L-1 SNP in Transgenic birch, the effect of SNP(sodium nitroprusside) on the expression of exogenous BGT, methyltransferase gene DRM, MET and the level of DNA methylation were determined in transgenic birch callus. The activities of defense enzymes and the MDA(malondialdehyde) content in transgenic birch were increased significantly, which indicated high concentration of NO had harm on the normal life of birch cells. The methyltransferase DRM and MET genes were up-regulated and the methylation level of genomic DNA was increased from 10.6% to 16.5%. The transcription level of BGT was improved at 6 h and only 0.46 folds of the control at 3 d, which suggested the expression of exogenous BGT gene in transgenic brich was affected by high concentration of NO and the level of genomic methylation. The results revealed the response patterns of exogenous gene and methyltransferase gene and determined the genomic methylation level and physiological and biochemical characteristics to the high concentration of NO in birch, which will provide some references for further study of epigenetic regulation and regulation of exogenous gene expression in transgenic plants.

Key words: NO, transgenic birch, gene expression, methylation

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