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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (4): 613-620.doi: 10.7525/j.issn.1673-5102.2017.04.018

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Response Surface Optimization of Culture Conditions for the Formation of Early Embryos in Larix olgensis

SONG Yue, LI Shu-Juan, BAI Xiao-Ming, ZHANG Han-Guo   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2017-01-06 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    National High-tech R&D Program("863" Program 2013AA102704-04-03)

Abstract: With the larch embryogenic callus, through the Design Box-Behnken design test, we established a response surface model based on the somatic embryogenesis. The culture conditions of somatic embryo early maturation was optimized in Larix olgensis. The relative ion concentration(P=0.007), the inositol concentration(P=0.000), the culture period(P=0.000) and the interaction between inositol content and culture period(P=0.007) effect on the number of somatic embryos of L.olgensis were extremely significant. Cultivation of Larix somatic embryo maturation was 337.04 ind·g-1 at the early stage of the most optimum conditions for relative ion content of 26.767 7% BM basic culture medium, inositol 10.454 5 g·L-1, culture time 12.656 6 d, and the number of somatic embryos average. In order to facilitate the practical operation, the culture condition was changed to the relative ion concentration of 25% BM, inositol 10 g·L-1, incubation period of 13 d, then the average number of somatic embryos was 336.46 ind·g-1, that was consistent with the predicted value 336.29 ind·g-1. Compared with previous test, the quantity and quality of L.olgensis somatic embryos were increased significantly.

Key words: Larix, Larix olgensis, response surface optimization, somatic embryogenesis, early embryo formation

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