Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (2): 259-265.doi: 10.7525/j.issn.1673-5102.2017.02.014

Previous Articles     Next Articles

Genetic Diversity and Fingerprints with SCoT Markers in Actinidia

ZHANG An-Shi1, ZHANG Zhong-Hai1, QI Xiu-Juan2, LIU Ying1, LUO Yang1   

  1. 1. School of Science, Jiaozuo Teachers College, Jiaozuo 454000;
    2. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
  • Received:2016-11-07 Online:2017-03-15 Published:2017-03-11
  • Supported by:
    Special funds for science and technology innovation project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2016-ZFRI)

Abstract: SCoT were used to analysis genetic diversity of the samples of 32 Actinidia varieties. The 11 primers were screened from 47 SCoT primers and 185 SCoT bands were obtained, including 180 polymorphic bands, with a polymorphism rate of 97.30%. The average Nei's gene diversity(H) and Shannon's information index(I) were 0.2384 and 0.3778. The cluster analysis conducted with UPGMA showed that 32 Actinidia varieties were divided into 5 groups at the genetic similarity coefficient of 0.78, and the constructed phylogenetic trees based on SCoT were consistent with the morphology results. The DNA fingerprints for 32 Actinidia varieties were established with 16 sites from 4 SCoT primers, and 32 Actinidia varieties could be identified by SCoT fringerprints.

Key words: Actinidia, SCoT, DNA fingerprint, genetic diversity

CLC Number: