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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (1): 63-68.doi: 10.7525/j.issn.1673-5102.2017.01.009

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Expression and Stress Tolerance Charaterization of a ThDREB Gene from Tamarix hispida in Yeast

FENG De-Ming1, WEN Pei-Ying1, ZHAO Chang1, YANG Yuan-Biao2, YANG Gui-Yan1, YU Li-Li1, GAO Cai-Qiu1   

  1. 1. Northeast Forestry University, State Key Laboratory of Tree Genetics and Breeding, Harbin 150040;
    2. The Maoershan Teaching Area of Northeast Forestry University, Shangzhi 150040
  • Received:2016-06-21 Online:2017-01-15 Published:2017-03-06
  • Supported by:
    National-level College Students' Innovative Entrepreneurial Training Plan Program(20151022507) and the Scientific Research Fund of Heilongjiang Provincial Education Department(No.12523017).

Abstract: DREB protein could specifically bind to DRE(dehydration responsive element) cis-acting elements, and regulate the expression of downstream stress related gene. It plays an important role in the regulation responses of plants to abiotic stress. To explore the abiotic stress tolerance role of DREB(ThDREB) from Tamarix hispida, the recombinant plasmid(named as pYES2-ThDREB) was constructed by inserting the ThDREB gene into the yeast expression vector pYES2. The plasmid pYES2-ThDREB was transformed into the yeast Saccharomyces cerevisiae, and the yeast transformed with empty pYES2 was used as the control. The pYES2-ThDREB and pYES2 recombinant yeast were treated with sorbitol, H2O2, CdCl2, NaCl, Na2CO3, MgCl2, and -20℃, respectively. The pYES2-ThDREB recombinant yeast was tolerant to all above abiotic treatments, suggesting that ThDREB maybe an important stress-regulating genes in T.hispida.

Key words: Tamarix hispida, DREB, yeast expression, abiotic stress tolerance

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