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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (5): 775-781.doi: 10.7525/j.issn.1673-5102.2016.05.020

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Characterization of Microsatellites in Genome of Pinus squamata, a Critically Endangered Species in the World

RUAN Zhen-Yuan1, WANG Bing-Yi2, OUYANG Zhi-Qin3, LIAO Li-Bin2, SU Teng-Wei1, QIAO Lu1   

  1. 1. Department of Landscape Architecture, Yunnan Forestry Technologicl College, Kunming 650224;
    2. The Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224;
    3. Yunnan Institute of Environmental Science, Kunming 650034
  • Received:2016-03-16 Online:2016-09-15 Published:2016-09-27
  • Supported by:
    Science Research Fund of Yunnan Provincial Education Department(2014Y569);Special Fund for the Protection of Biological Diversity in Yunnan(2013-004);Doctoral Program of Yunnan Forestry Technological College(KY(BS)201402);Science Research Fund of Yunnan Provincial Education Department(2014Y571)

Abstract: Pinus squamata is a critically endangered species in the world, therefore, developing simple sequence repeat(SSR) primers will facilitate the species genetic research and conservation. The complete genome sequence of P.squamaia was determined through Illumina genome analyzer, and 2 651 microsatellites were discovered by MISA software. Single nucleotide repeat number occupied the highest proportion of these microsatellites which may indicate a long evolution history of P.squamata. Among different repeat types, A/T contents was significantly higher than G/C contents. In terms of distinct length repeat units, the length of dinucleotide repeat microsatellite varies with the highest degree. The length of SSRs with different repeat type was negatively correlated with frequency of occurrences. The available microsatellite sequences can satisfy P.squamaia population genetics, and reveal the species bias and corresponding potential functions.

Key words: Pinus squamata X.W.Li, genome, microsatellite

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