Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (5): 730-738.doi: 10.7525/j.issn.1673-5102.2016.05.014

Previous Articles     Next Articles

Bud Inducing from 16-year Elite Trees Explants of Three Acacia Species

WANG Hong, HUANG Lie-Jian, HU Feng   

  1. Research Institute of Tropical Forestry, CAF, Guangzhou 510520
  • Received:2016-03-22 Online:2016-09-15 Published:2016-09-27
  • Supported by:
    12th-Five-Year National Science and Technology Support Program of China(2012BAD01B0402)

Abstract: The explants from 16-year elite trees of Acacia mangium, A.auriculiformis and A.mangium×A.auriculiformis were used to establish the efficient bud inducing technique, by studying the sterilization methods, position, gathered time and type of explants, the effects of 6-BA, sucrose, and basal medium. A.mangium was disinfectant sensitive, treatment with mercuric chloride 9 min and ethanol 15 s was the optimal, but longer time were need for A.auriculiformis and A.mangium×A.auriculiformis, treatment with mercuric chloride 18 min, ethanol 15 s and mercuric chloride 15 min, and ethanol 30 s, respectively. These three Acacia species shared common optimal position, gathered season and type:3-5th node of shoot as explants were optimal position. It was the optimal season to gather explants in August; Type 2 explant germinated from shoots of cutting orchard (established by cutting the shoots of mature elite) was the optimal type; The optimal bud inducing medium were modify MS+6-BA 0.5 mg·L-1+sucrose 30 g·L-1 for A.mangium, MS+6-BA 1.0 mg·L-1+sucrose 40 g·L-1 for A.auriculiformis, MS+ sucrose 20 g·L-1 for A.mangium×A.auriculiformis, with the highest germination rate of 93.33%, 92.00% and 97.33%, respectively.

Key words: Acacia mangium, A.auriculiformis, A.mangium×, A.auriculiformis, explants, bud inducing

CLC Number: