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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (4): 627-633.doi: 10.7525/j.issn.1673-5102.2016.04.020

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Extraction Process Optimization of Inulin from Helianthus tuberosus L. by Response Surface Methodology

LIU Yan1,2, CHEN Xiao-Yin1, YANG Li-Li1, XIE Rui1, HE Nan1, ZHANG Ji1,2   

  1. 1. College of Life Science, Northwest Normal University, Lanzhou 730070;
    2. Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070
  • Received:2015-11-03 Online:2016-07-15 Published:2016-06-15
  • Supported by:
    National Key Technology R&D Program of the Ministry of Science and Technology of China

Abstract: Single-factor tests and response surface method (RSM) were used to optimize the extraction process of Helianthus tuberosus L. inulin in tubers.In order to obtain the maximum of extraction yield of the inulin, three main factors including liquid-solid ratio, extraction times and extraction temperature were selected with the Design Expert V8.0 design, and a quadric regression equation for predicting the yield of inulin was established. The results showed that the optimum extraction conditions were as follows:liquid-solid ratio of 18:1(mL·g-1), extraction time of 41 min and extraction temperature of 85℃. Under the conditions, the optimum extracting yield of inulin was 40.56%, which is well matched with the predictive extracting yield of 40.74%. Under the process, we carried out the rough inulin and analyzed extraction of the inulin by infrared spectrum with the simple and low cost process.

Key words: Helianthus tuberosus L., inulin, response surface methodology, extraction process

CLC Number: