Responses of Main Secondary Metabolites and DPPH Free Radical Scavenging Activity of the Korean Pine to Drought Stress

doi:10.7525/j.issn.1673-5102.2016.04.009

Abstract

Abstract: The Korean pine were tested to study the effects of drought stress and rehydration on flavonoids content, tannin content, procyanidin content and DPPH free radical scavenging activity of needles and bark chlorenchyma. Flavonoids, tanninand procyanidin content of bark chlorenchyma decreased significantly under drought stress, flavonoids and tannin content continue to decrease after rehydration, and three kinds of content were not returned to control levels. Flavonoids and procyanidin content of needles decreased and tannin content significantly increased, after rehydration they were not returned to control levels. IC₅₀ values of needles and bark chlorenchyma significantly increased under drought stress, and DPPH free radical scavenging activity of extract was significantly decreased, after rehydration DPPH free radical scavenging activity of needle and bark chlorenchyma were returned to control levels. Secondary metabolites and antioxidant activity of needles and bark chlorenchyma were significantly affected by drought stress, but there were some different responses to drought stress between needles and bark chlorenchyma.

Key words: drought stress, needles, bark chlorenchyma, flavonoids, tannin, procyanidin, DPPH free radical

CLC Number:

S791.247

ZHANG Dan, REN Jie, LIU Hong-Mei, WANG Hui-Mei. Responses of Main Secondary Metabolites and DPPH Free Radical Scavenging Activity of the Korean Pine to Drought Stress[J]. Bulletin of Botanical Research, 2016, 36(4): 542-548.

References

1.Chen J W,Zhang Q,Li X S,et al.Gas exchange and hydraulics in seedlings of Hevea brasiliensis during water stress and recovery[J].Tree Physiology,2010,30(7):876-885.
2.Shao H B,Chu L Y,Jaleel C A,et al.Understanding water deficit stress-induced changes in the basic metabolism of higher plants-biotechnologically and sustainably improving agriculture and the eco-environment in arid regions of the globe[J].Critical Reviews in Biotechnology,2009,29(2):131-151.
3.Shao H B,Chu L Y,Shao M A.Calcium as a versatile plant signal transducer under soil water stress[J].Bio Essays,2008,30(7):634-641.
4.Dicko M H,Gruppen H,Barro C,et al.Impact of phenolic compounds and related enzymes in sorghum varieties for resistance and susceptibility to biotic and abiotic stresses[J].Journal of Chemical Ecology,2005,31(11):2671-2687.
5.Oh M M,Trick H N,Rajashekar C B.Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce[J].Journal of Plant Physiology,2009,166(2):180-191.
6.Ghahfarokhi M G,Mansurifar S,Taghizadeh-mehrjardi R,et al.Effects of drought stress and rewatering on antioxidant systems and relative water content in different growth stages of maize(Zea mays L.) hybrids[J].Archives of Agronomy and Soil Science,2015,61(4):493-506.
7.Paulsen J,Kleinwaechter M,Selmar D,et al.Beneficial impacts of drought stress on the contents of secondary metabolites in plant-derived commodities[J].Journal of Medicinal & Spice Plants,2014,19(4):193-195.
8.阎秀峰,李晶,祖元刚.干旱胁迫对红松幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,1999,19(6):850-854.
Yan X F,Li J,Zu Y G.Effect of drought stress on activity of cell defense enzymes and lipid peroxidation in korean pine seedlings[J].Acta Ecologica Sinica,1999,19(6):850-854.
9.吕海亮,毛子军,李娜.干旱对红松和蒙古栎幼苗生长的影响及种间竞争[J].植物研究,2014,34(3):364-371.
Liu H L,Mao Z J,Li N.Response of growth and interspecific competition of Pinus koraiensis and Quercus mongolica seedlings to drought[J].Bulletin of Botanical Research,2014,34(3):364-371.
10.金微微,王炎,张会慧,等.茉莉酸甲酯对干旱及复水条件下烤烟幼苗叶绿素荧光特性的影响[J].应用生态学报,2011,22(12):3157-3162.
Jin W W,Wang Y,Zhang H H,et al.Effects of foliar spraying methyl jasmonate on leaf chlorophyll fluorescence characteristics of flue-cured tobacco seedlings under drought and re-watering[J].Chinese Journal of Applied Ecology,2011,22:3157-3162.
11.马富举,李丹丹,蔡剑,等.干旱胁迫对小麦幼苗根系生长和叶片光合作用的影响[J].应用生态学报,2012,23(3):724-730.
Ma F J,Li D D,Cai J,et al.Responses of wheat seedlings root growth and leaf photosynthesis to drought stress[J].Journal of Applied Ecology,2012,23:724-730.
12.吕欣,付玉杰,王微,等.紫外分光光度法测定甘草黄酮含量[J].植物研究,2003,23(2):192-194.
Lü X,Fu Y J,Wang W,et al.Determination of flavonoids in Glycyrrhiza uralensis fisch with ultraviolet spectrophotometry[J].Bulletin of Botanical Research,2003,23(2):192-194.
13.武予清,郭予元.棉花植株中的单宁测定方法研究[J].应用生态学报,2000,11(2):243-245.
Wu Y Q,Guo Y Y.Determination of tannin in cotton plant[J].Chinese Journal of Applied Ecology,2000,11(2):243-245.
14.Deshpande S S,Cheryan M.Determination of phenolic compounds of dry beans using vanillin,redox and precipitation assays[J].Journal of Food Science,1987,52(2):332-334.
15.段琦梅,梁宗锁,聂小妮,等.黄芪和党参提取物的抗氧化活性研究[J].西北植物学报,2010,30(10):2123-2127.
Duan Q M,Liang Z S,Nie X N,et al.Antioxidant activity detection of Astragalus membranaceus and Codonopsis pilosula ethanol extracts by DPPH method[J].Acta Bot Boreal-Occident Sin,2010,30(10):2123-2127.
16.Wahid A,Gelani S,Ashraf M,et al.Heat tolerance in plants:An overview[J].Environmental and Experimental Botany,2007,61(3):199-223.
17.Castellarin S D,Pfeiffer A,Sivilotti P,et al.Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit[J].Plant,Cell & Environment,2007,30(11):1381-1399.
18.阎秀峰,王洋,李一蒙.植物次生代谢及其与环境的关系[J].生态学报,2007,27(6):2554-2562.
Yan X F,Wang Y,Li Y M.Plant secondary metabolism and its response to environment[J].Acta Ecologica Sinica,2007,27(6):2554-2562.
19.Zahir A,Abbasi B H,Adil M,et al.Synergistic effects of drought stress and photoperiods on phenology and secondary metabolism of Silybum marianum[J].Applied Biochemistry and Biotechnology,2014,174(2):693-707.
20.程春龙,刘松,廖容苏,等.额济纳绿洲胡杨(Populus euphratica)酚类物质含量和分布及其与土壤水分的关系[J].生态学报,2008,28(1):69-75.
Cheng C L,Liu S,Liao R S,et al.Concentration and distribution of phenolic compounds in Populus euphratica in Ejina oasis and their correlation with soil water contents[J].Acta Ecologica Sinica,2008,28(1):69-75.
21.Alonso-amelot M E,Oliveros-bastidas A,Calcagno-pisarelli M P.Phenolics and condensed tannins of high altitude Pteridium arachnoideum in relation to sunlight exposure,elevation,and rain regime[J].Biochemical Systematics and Ecology,2007,35(1):1-10.
22.Cobb N S,Mopper S,Gehring C A,et al.Increased moth herbivory associated with environmental stress of pinyon pine at local and regional levels[J].Oecologia,1997,109(3):389-397.
23.杨蓓芬,金则新,邵红,等.七子花不同器官次生代谢产物含量的分析[J].植物研究,2007,27(2):229-232.
Yang P F,Jin Z X,Shao H,et al.Analysis of secondary metabolites contents in the different organs of Heptacodium miconioides[J].Bulletin of Botanical Research,2007,27(2):229-232.
24.Said E M,Mahmoud R A,Al-akshar R,et al.Drought stress tolerance and enhancement of banana plantlets in vitro[J].Austin Journal of Biotechnology & Bioengineering,2015,2(2):1040.
25.Rebey I B,Jabri-karoui I,Hamrouni-sellami I,et al.Effect of drought on the biochemical composition and antioxidant activities of cumin(Cuminum cyminum L.) seeds[J].Industrial Crops and Products,2012,36(1):238-245.

[1]	Zhezhe LI, Yidan ZHANG, Bo WANG, Zhenghao WANG, Lu WANG, Beibei CHENG. Changes and Influencing Factors of Anthocyanins in Hibiscus syriacus During Flowering [J]. Bulletin of Botanical Research, 2023, 43(4): 550-561.
[2]	Na LI, Xiaonan WANG. Screening and Phenotypic Identification of Seed Coat Pigmentation Mutants in Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2023, 43(1): 59-68.
[3]	Mengqiao GUO, Xuanyu CHEN, Senrong HONG, Jiao LI, Jie FAN, Xinyu CHENG. The Correlation Between Leaf Phenotype Diversity and Total Flavonoids Content of Overground Part of Tetrastigma hemsleyanum Diels & Gilg [J]. Bulletin of Botanical Research, 2022, 42(5): 876-885.
[4]	Bin WEI, Yi LI, Shiping SU. The Effect of Exogenous Proline on the Stomata of Nitraria tangutorum Leaves under Natural Drought [J]. Bulletin of Botanical Research, 2022, 42(3): 492-501.
[5]	Feng HE, Hong-Yan DU, Pan-Feng LIU, Lu WANG, Jun QING, Lan-Ying DU. Effects of Drought Stress on Leaf Structure of Eucommia ulmoides [J]. Bulletin of Botanical Research, 2021, 41(6): 947-956.
[6]	Shuang-Hui TIAN, He CHENG, Yang ZHANG, Cong LIU, De-An XIA, Zhi-Gang WEI. Genome-wide Identification and Expressional Analysis of Carotenoid Cleavage Dioxygenases(CCD) Gene Family in Populus trichocarpa under Drought and Salt Stress [J]. Bulletin of Botanical Research, 2021, 41(6): 993-1005.
[7]	Dong ZHANG, Yan LIU, Han ZHANG, Zi-Jian ZHANG, Yang WANG, Mei-Cen LIU. Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis [J]. Bulletin of Botanical Research, 2021, 41(3): 449-457.
[8]	Xiao-Chi YU, Gui-Juan YANG, Ju-Lan DONG, Jun-Hui WANG, Wen-Jun MA, Peng ZHANG. Physiological Responses to Drought Stress of Five Speciesfrom Catalpa Scop [J]. Bulletin of Botanical Research, 2021, 41(1): 44-52.
[9]	Wen-Hai HU, Xiao-Hong YAN, Xiao-Hong LI, Zao-Gui CAO. Effects of 24-Epibrassinolide on the Chlorophyll Fluorescence Transient in Leaves of Pepper under Drought Stress [J]. Bulletin of Botanical Research, 2021, 41(1): 53-59.
[10]	Yong-Mei MIAO, Yuan Tong, Da Fang, Yu-Hao Wang. Effects of Rare Earth Element Lanthanum on Adventitious Buds Induction, Plantlets Growth and Synthesis of Secondary Metabolites of Dendrobium officinale [J]. Bulletin of Botanical Research, 2020, 40(6): 839-845.
[11]	FANG Zi-Wen, ZHANG Xia-Yan, TAO Jun, ZHAO Da-Qiu. Ameliorative Effect of Ferulic Acid on Paeonia ostii under Drought Stress [J]. Bulletin of Botanical Research, 2020, 40(3): 353-359.
[12]	QIAO Bin-Jie, WANG De-Qiu, GAO Hai-Yan, LI Zhao-Min, GE Li-Li, DING Wen-Ya, ZHAO Xi-Yang. Photosynthetic and Stomatal Morphological Variation of Poplar Clones in Seedling Stage under Drought Stress [J]. Bulletin of Botanical Research, 2020, 40(2): 177-188.
[13]	ZHAO Min, HAO Wen-Ying, NING Xin-Zhe, HAO Long-Fei, YAN Hai-Xia, MU Ya-Nan, BAI Shu-Lan. Screening of Excellent Ectomycorrhizal Fungi-tree for Drought Resistant with Pinus sylvestris var. mongolica [J]. Bulletin of Botanical Research, 2020, 40(1): 133-140.
[14]	JI Li, HAN Jiao, WANG Fang, WANG Jun, SONG Di, ZHANG Li-Jie, QI Yong-Hui, YANG Yu-Chun. Effects of Drought Stress on Photosynthetic and Physiological Characteristics of Juglans mandshurica Seedlings in Different Soil Substrates [J]. Bulletin of Botanical Research, 2019, 39(5): 722-732.
[15]	JIN Jiao-Jiao, SONG Zi-Wen, MA Xiao-Yu, SUN Guo-Yu, LI Kai-Long. Morphological and Physiological Responses of Populus ussuriensis Kom. to Drought Stress [J]. Bulletin of Botanical Research, 2019, 39(4): 490-496.