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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (2): 242-251.doi: 10.7525/j.issn.1673-5102.2016.02.013

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Allelic Variation in 4-coumarate:CoA ligase(4CL) Associated with Wood Properties of Larix olgensis

WANG Yan-Hong1,2;JIA Qing-Bin1;ZHANG Lei1;ZHANG Han-Guo1*   

  1. 1.Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Northeast Forestry University,Harbin 150040;
    2.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030
  • Online:2016-03-15 Published:2016-05-20

Abstract: We used 10 provenances of Larix olgensis to study the effects of polymorphism of 4CL gene on wood quality and growth traits in L.olgensis. The polymorphism in 4CL gene was identified using DNA sequencing, 64 mean SNPs were identified,the median SNP frequency was one site per 31 bp,and the average nucleotide diversity for the sequenced regions was calculated to be πT =0.015 77 and θW =0.008 78. The diversity level of synonymous nucleotide sbustituons(πsyn=0.022 56) was 1.4 of nonsynoymous nucleotide substitutions(πnonsyn=0.015 75), suggesting that the gene misht be evolved under purifying selection at the synonoymous sites of the coding region in L.olgensis. The association between the 27 common SNPs(frequency>10%), haplotypes and wood quality and growth traits were analyzed respectively, indicating that 7 SNPs and 6 haplotypes were significantly associated with wood quality and growth traits, explaining between 0.09%-2.08% of the phenotypic variance. Ourstudy provided an important genetic foundation for molecular marker-assistant selection breeding programs with the goals of improving the wood quality and growth products in L.olgensis.

Key words: Larix olgensis, lignin, 4CL gene, SNPs, haplotype