Studies on Dynamic Change of Alkaloids Contents and Expression of the Associated Biosynthetic Genes in Catharathus roseus Leaves

doi:10.7525/j.issn.1673-5102.2015.05.018

Abstract

Abstract: Catharanthus roseus seedlings of 45d were grown in water-culture. The different PEG6000 concentration(15%, 20%, 25%, 30%, 35%, 40%(V/V)were set to treat seedlings in greenhouse. The effect of on the proline content and the relative water content(RWC) of leaves were determineted, 35% PEG6000 concentration was selected. On the first 12-24 h hours under 35% PEG6000 treatment, the POD activities were increased gradually compared to control. High performance liquid chromatography analysis showed, there were gradual increase and then decrease in the contents of vindoline(VIN) and catharanthine(CAT) under stress, but the content of VBL increased gradually and reached the maximum on the 24 h hour of treatment. In addition, the data from the SQ-RT-PCR determination indicated that the genes(Tdc, Str, Dat) expression level were higher than the control on the 3th hour of treatment. The further correlation analysis indicated that CAT accumulation was significantly correlated with Tdc gene expression, and VBL accumulation was significantly correlated with POD activities(P<0.05).

Key words: Catharanthus roseus, alkaloids, gene expression

CLC Number:

LIU Ying；LI De-Wen；MENG Qing-Huan；ZHANG Zhong-Hua；DUAN Xi-Hua*. Studies on Dynamic Change of Alkaloids Contents and Expression of the Associated Biosynthetic Genes in Catharathus roseus Leaves[J]. Bulletin of Botanical Research, 2015, 35(5): 746-750.

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