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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (3): 457-461.doi: 10.7525/j.issn.1673-5102.2015.03.021

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A Simple and Efficient Method of DNA Extraction from Different Plant Samples

DING Hao;ZHENG Tang-Chun;LIANG De-Yang;QU Guan-Zheng*   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Online:2015-05-20 Published:2015-06-24
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Abstract: It is difficult to extract high quality DNA from plants for molecular work, since there are a great deal of secondary metabolities. We provided a simple improved CTAB protocol for extracting plant DNA. RNA was digested with RNase A after the step of incubation at 65℃. Then, DNA was extracted, precipitated and washed. The purity and quality of total DNA was identified by agarose gel electrophoresis and nucleic acid detection instrument. PCR amplification of the extracted DNAs as templates could present clear bands of the target gene. Compared with the previous methods, it was simple and high throughput to be used for extraction of purified DNA from different plant samples, which would set a technical foundation of subsequent analysis of molecular biology.

Key words: plant, improved method, extraction of DNA, PCR

CLC Number: