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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (3): 340-346.doi: 10.7525/j.issn.1673-5102.2015.03.004

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Cloning and Identification of a Novel thioredoxin h Gene from Tamarix hisipida

SONG Xin1;DONG Jing-Xiang2;LI Kai-Long1;LI Hong-Jiao1;LI Hui-Yu1*   

  1. 1.State Key Laboratory of Forest Genetics and Tree Breeding ,Northeast Forestry University,Harbin 150040;
    2.Library,Northeast Forestry University,Harbin 150040
  • Online:2015-05-20 Published:2015-06-24
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Abstract: Thioredoxins play an important role in various cellular processes through redox regulation. We isolated a novel Trx cDNA sequence, ThTrx1, from cDNA libraries of T.hisipida to characterize their functions under abiotic stress. By bioinformatic analysis, ThTrx1 is a new member of the thioredoxin h subfamily and belongs to subgroup 1. We used real-time quantitative RT-PCR to examine ThTrx1 gene expression in roots and leaves under different abiotic stresses(NaCl, PEG, CdCl2, low temperature and ABA). The mRNA levels of ThTrx1 in both leaves and roots were up-regulated under NaCl, PEG, CdCl2 and ABA treatments. However, the expression of ThTrx1 mRNA decreased during low temperature treatment. We also expressed the ThTrx1 gene in E.coli and examined its effect on bacterial growth and stress tolerance. The E.coli containing recombinant plasmid(pET32a-Trx1) was more resistant to salt, metal ions and drought stress that was the control. Therefore, ThTrx1 is involved in an abiotic stress response and regulated by ABA-dependent signaling pathways.

Key words: Thioredoxin h, gene expression, abiotic stress response, Tamarix hisipida

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