Optimization of SRAP-PCR System for Opisthopappus taihangensis(Ling) Shih by Uniform Design

doi:10.7525/j.issn.1673-5102.2014.05.020

Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (5): 712-715.doi: 10.7525/j.issn.1673-5102.2014.05.020

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Optimization of SRAP-PCR System for Opisthopappus taihangensis(Ling) Shih by Uniform Design

ZHANG An-Shi;LIU Ying;ZHANG Wei-Min;ZHAO Li-Xin

1.Department of Biology,Jiaozuo Teachers College,Jiaozuo　454003；2.Henan Province Jiaozuo National Forestry Plant,Jiaozuo　454191

Received:1900-01-01 Revised:1900-01-01 Online:2014-09-20 Published:2015-03-19
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Abstract

Abstract: In the present study, SRAP-PCR reaction conditions for Opisthopappus taihangensis(Ling) Shih were optimized. The factors affecting SRAP results of O.taihangensis were investigated by uniform designs of two times. The optimal protocol was accomplished in 10 μL reaction volumes containing 1.3 μL template DNA, 0.35 μL primers and 5.3 μL 2×Taq MasterMix. Under this optimal condition, clear and polymorphic bands from 11 O.taihangensis plants had been obtained using Me1/em4 primers. The result showed that the reaction condition was suitable for SRAP-PCR reaction system of O.taihangensis.

Key words: Opisthopappus taihangensis, SRAP, uniform design, optimization

CLC Number:

ZHANG An-Shi;LIU Ying;ZHANG Wei-Min;ZHAO Li-Xin. Optimization of SRAP-PCR System for Opisthopappus taihangensis(Ling) Shih by Uniform Design[J]. Bulletin of Botanical Research, 2014, 34(5): 712-715.

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Optimization of SRAP-PCR System for Opisthopappus taihangensis(Ling) Shih by Uniform Design

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