Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (5): 712-715.doi: 10.7525/j.issn.1673-5102.2014.05.020

Previous Articles     Next Articles

Optimization of SRAP-PCR System for Opisthopappus taihangensis(Ling) Shih by Uniform Design

ZHANG An-Shi;LIU Ying;ZHANG Wei-Min;ZHAO Li-Xin   

  1. 1.Department of Biology,Jiaozuo Teachers College,Jiaozuo 454003;2.Henan Province Jiaozuo National Forestry Plant,Jiaozuo 454191
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-09-20 Published:2015-03-19
  • Supported by:

Abstract: In the present study, SRAP-PCR reaction conditions for Opisthopappus taihangensis(Ling) Shih were optimized. The factors affecting SRAP results of O.taihangensis were investigated by uniform designs of two times. The optimal protocol was accomplished in 10 μL reaction volumes containing 1.3 μL template DNA, 0.35 μL primers and 5.3 μL 2×Taq MasterMix. Under this optimal condition, clear and polymorphic bands from 11 O.taihangensis plants had been obtained using Me1/em4 primers. The result showed that the reaction condition was suitable for SRAP-PCR reaction system of O.taihangensis.

Key words: Opisthopappus taihangensis, SRAP, uniform design, optimization

CLC Number: