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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (2): 245-251.doi: 10.7525/j.issn.1673-5102.2014.02.017

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Cloning and Expression Analysis of Cyclophilins A(CyPA) Gene form Chlorella

WANG Xu-Hui;QIAO Kun;WANG Zhen-Juan;GUAN Qing-Jie*;LIU Shen-Kui   

  1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field(SAVER),Ministry of Education,Alkali Soil Natural Environmental Science Center(ASNESC),Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-03-20 Published:2014-03-20
  • Contact: GUAN Qing-Jie
  • Supported by:

Abstract: Cyclophilins (CyPs) are widely distributed in organisms, and play important roles. Cyclophilin A-like was screened in our study from Chlorella sp. cDNA library. By extracting Arabidopsis thaliana protoplasts that was transformed the 35S::CyPA::GFP fusion plasmid, it showed that CyPA located in the chloroplasts. Overexpression of CyPA gene in yeast enhanced the InV yeast growth ability to resistance of NaCl, NaHCO3 and drought stress. Real-time quantitative PCR showed that under abiotic stress conditions, CyPA gene expression quantity increased. So we speculated that CyPA genes were associated with abiotic stress resistance, through the cyclophilin A involved in improving survival ability of resistant organisms.

Key words: chlorella, cyclophilins, real time quantitative PCR, expression pattern of gene

CLC Number: