Abstract: A novel germin-like protein gene clone was analyzed from a cDNA library of Sasussured involucrate, named SikGLP. Bioinformatics analysis showed that the SikGLP gene contains a 633 bp open reading frame encoding 210 amino acids with a theoretical molecular mass of 21.6 kDa and an isoelectric point of 6.81. SikGLP was a hydrophobic protein and contained a signal peptide. Multiple sequence alignment of GLP proteins revealed that the GLP gene shares high identities in sequence, and possesses typical characteristics of plant germin-like proteins. Phylogenetic analysis among 17 species indicated that SikGLP was clustered with Vitis vinifera. Real time-PCR analysis showed that the expression of SikGLP gene by low temperature, MV and PEG stresses. It may plays a role in improving resistance capacity of early stress.

Key words: Sasussured involucrata Kar.et Kir, germin-like proteins, real time-PCR