Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (1): 66-72.doi: 10.7525/j.issn.1673-5102.2013.01.011

Previous Articles     Next Articles

Cloning and Expression Analysis of Tea Hydroperoxide Lyase Gene CsiHPL1

XIN Zhao-Jun;SUN Xiao-Ling;ZHANG Zheng-Qun;CHEN Zong-Mao*   

  1. Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-01-20 Published:2013-01-20
  • Contact: CHEN Zong-Mao
  • Supported by:

Abstract: Primers were designed according to the cDNA sequence of tea CsiHPL1. RT-PCR method was used to clone CsiHPL1 from Longjing 43′. The expression of CsiHPL1 under biotic and abiotic stress and subcellular localization were analyzed. The results showed CsiHPL1 contains an open reading frame of 1 476 bp which encodes a protein of 491 amino acids. This gene was predicted as a 13-HPL gene. Tea geometrid feeding, wounding and JA treatment upregulated the expression levels of CsiHPL1. The total sequence of this gene was fused with GFP to construct a binary vector for tobacco transient transformation. Under confocal laser-scanning microscopy, green fluorescent signals were localized in chloroplasts in transgenic tobacco plants, suggesting that the gene encodes a protein targeting to chloroplast.

Key words: tea, hydroperoxide lyase gene, clone, expression

CLC Number: