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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (6): 756-759.doi: 10.7525/j.issn.1673-5102.2012.06.021

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Microwave-assisted Extraction of Genistein from Pigeon Pea[Cajanus cajan(L.) Millsp.] Roots

PENG Xiao;DUAN Ming-Hui;PAN You-Zhi;ZHANG Dong-Yang;DUAN Xi-Hua;*   

  1. 1.State Engineering Laboratory for Bio-resource Eco-utilization,Northeast Forestry University,Harbin 150040;2.Engineering Research Center of Forest Bio-prepration,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Contact: DUAN Xi-Hua
  • Supported by:

Abstract: Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study microwave-assisted extraction of genistein from the roots of pigeon pea. The optimum parameters were as follows: extraction temperature 68 ℃, liquid/solid ratio 32∶1 mL·g-1, ethanol concentration 78%, extraction power 700 W and extraction time 15 min. Under the optimal conditions, the average extraction yield of genistein was 0.465±0.032 mg·g-1. This study is significant for the application of microwave extraction technology and utilization of the pigeon pea root.

Key words: pigeon pea roots, genistein, microwave-assisted extraction, Box-Behnken design

CLC Number: