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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 497-500.doi: 10.7525/j.issn.1673-5102.2012.04.021

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Ultrasound-assisted Extraction of 2″-O-galloylhyperin from Pyrola calliantha H.Andr.

YAO Xiao-Hui;DUAN Xi-Hua;*;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040；2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: DUAN Xi-Hua
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Abstract: Based on the preliminary experiments, a three-variable, three-level Central Composite Design (CCD) was performed to study the ultrasound-assisted extraction of 2″-O-galloylhyperin from Pyrola calliantha H.Andr.. The optimum parameters were as follows: extraction time 45 min, ultrasound power 40 kHz, three extraction cycles, extraction temperature 60℃, liquid/solid ratio 33∶1, ethanol concentration 50%. Under the optimal conditions, the average extraction yields of 2″-O-galloylhyperin was 5.024 mg·g-1.

Key words: Pyrola calliantha H.Andr., 2&Prime, -O-galloylhyperin, ultrasound-assisted extraction, Central Composite Design

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