Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 484-487.doi: 10.7525/j.issn.1673-5102.2012.04.018
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GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;*
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Abstract: The seeds of Sinopodophyllum hexandrum Royle were taken as materials. The effects of exogenous hormones on induction, multiplication and rooting of explants were discussed. The regeneration system of S.hexandrum has been established. The results showed that: The optimal conditions for shoot induction were 3.0 mg·L-1 6-BA+0.5 mg·L-1 GA-3. The induction rate was 85.71% with a shortening of 30-40 d. For multiplication, the optimal conditions were 3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA, and the mean shoots obtained per explant was 1.63. The most proper rooting medium was: WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA and the rooting ratio was 60.1% after 30 d culturing.
Key words: Sinopodophyllum hexandrum Royle, seeding, hormones, In vitro culture
CLC Number:
S567.2
GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;*. In vitro Culture of Endangered Medicinal Plant Sinopodophyllum hexandrum Royle[J]. Bulletin of Botanical Research, 2012, 32(4): 484-487.
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URL: http://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2012.04.018
http://bbr.nefu.edu.cn/EN/Y2012/V32/I4/484