Tumor Cell Absorption of Folate Conjugated Albumin Nanoparticle and Folate Receptor-related Gene Expression

doi:10.7525/j.issn.1673-5102.2012.03.019

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (3): 360-364.doi: 10.7525/j.issn.1673-5102.2012.03.019

Previous Articles Next Articles

Tumor Cell Absorption of Folate Conjugated Albumin Nanoparticle and Folate Receptor-related Gene Expression

NIE Si-Ming;ZHANG Zhong-Hua;ZHAO Dong-Mei;ZU Yuan-Gang*

Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040

Received:1900-01-01 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20
Contact: ZU Yuan-Gang
Supported by:

Abstract

Abstract: To investigate the effect of folate conjugated albumin nanoparticle on tumoral cell, the folate receptor positive FR(+) SK-OV-3 cell and FR(-) lung A549 cell were used for experiment. By the techniques of cell culture in vitro and semi QRT PCR, SKOV3 cell and A549 cell were treated by folate conjugated albumin nanoparticles in various concentrations for 24 h. Folate conjugated albumin nanoparticles significantly increased the expression of FR1 gene of SKOV3 cell. There was a positive correlation between the concentration of folate conjugated albumin nanoparticle and the expression of FR1 gene. Folate conjugated albumin nanoparticle increased the activity of FOLR1 protein and related gene, which may be one of important endocytosis mechanisms of folate conjugated albumin nanoparticle.

Key words: Folate, folate receptor, tumor cell, gene expression

CLC Number:

Q563+8

NIE Si-Ming;ZHANG Zhong-Hua;ZHAO Dong-Mei;ZU Yuan-Gang*. Tumor Cell Absorption of Folate Conjugated Albumin Nanoparticle and Folate Receptor-related Gene Expression[J]. Bulletin of Botanical Research, 2012, 32(3): 360-364.

[1]	HE Hao, ZHU Guo-Qing, CHEN Shi-Ya, XU Chang, JIN Shu-Mei. Cloning of LpPEX7 Gene from Lilium pumilum and Its Expression Characteristics under Salt Stress [J]. Bulletin of Botanical Research, 2020, 40(2): 274-283.
[2]	FAN Song-Le, WANG Ji-Kun, XIE Gui-Shui, WANG Meng, WANG Li-Feng. Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88 [J]. Bulletin of Botanical Research, 2020, 40(1): 106-116.
[3]	WANG Qi, XU Zhi-Ru, CHEN Jin-Yuan, ZHANG Shuang, HUANG Jia-Huan, LIU Guan-Jun. Identification and Expression Analysis of Heavy Metal-associated Isoprenylated Plant Proteins(HIPPs) Genes in Populus [J]. Bulletin of Botanical Research, 2019, 39(6): 935-946.
[4]	ZHANG Yue, ZHAO Xin, HOU Zheng, WANG Yan-Min, WANG Yu-Cheng, WANG Chao. Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(ThSAMS) Gene from Tamarix hispida [J]. Bulletin of Botanical Research, 2019, 39(1): 113-122.
[5]	SHAO Wan-Lu, LI Yue-Ling, GAO Song, LI Jun-Min, LIANG Zong-Suo. Effects of Light Intensity on the Fruit Coloration and Anthocyanian Biosynthesis in Fragaria×ananassa Duch. ‘Benihoppe’ and the Possible Molecular Mechanism [J]. Bulletin of Botanical Research, 2018, 38(5): 661-668.
[6]	SUN Hong, ZHAO Xing-Tang, LIU Zhang, YANG Kai, WANG Ying, ZHAN Ya-Guang. Bioinformatics of S6K Genes in Fraxinus mandshurica and Their Expression Analysis under Stress and Hormone [J]. Bulletin of Botanical Research, 2018, 38(5): 714-724.
[7]	ZHUO Wei, CHEN Qian, LUO Yin, YANG Shang-Yu, LU Li-Ming, LI Li-Qin. Cloning,Construction of Expression Vector and Expression Analysis of the Ethylene-responsive Factor ERF Gene NtTOE3 in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2018, 38(5): 733-740.
[8]	ZHANG Yu, ZHANG Yue, ZHANG Chun-Rui, WANG Yan-Min, WANG Yu-Cheng, WANG Chao. Cloning and Expression Analysis of S-Adenosine Methionine Decarboxylase(ThSAMDC) Gene from Tamarix ramosissima [J]. Bulletin of Botanical Research, 2018, 38(1): 132-140.
[9]	SUN Feng-Kun, LI Si-Da, LI Ji-Xiang, CHEN Xiao-Hui, ZENG Fan-Suo. Effects of Exogenous Nitric Oxide on Exogenous Gene Expression and DNA Methylation in Transgenic Birch [J]. Bulletin of Botanical Research, 2017, 37(6): 870-875.
[10]	LU Yan-Xi, ZHANG Dong, WANG Li-Feng, WANG Ji-Kun. Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg. [J]. Bulletin of Botanical Research, 2017, 37(6): 953-960.
[11]	DAI Li-Juan, ZHENG Tang-Chun, LIU Cai-Xia, LIU Yi, YOU Xiang-Ling, QU Guan-Zheng. Establishment of A Chemical-inducible Gene Expression System in Tobacco [J]. Bulletin of Botanical Research, 2016, 36(6): 917-924.
[12]	YANG Kai, SUN Ying-Kun, WANG Kui-Ling, LIU Qing-Hua, GAO Han-Dong. Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong) [J]. Bulletin of Botanical Research, 2016, 36(5): 753-759.
[13]	JIA Yuan-Yuan, ZHANG Chun-Rui, WANG Yu-Cheng, YANG Chuan-Ping, WANG Chao. Cloning and Expression Analysis of a Plasma Membrane Na⁺/H⁺ Antiporter Gene in Tamarix hispida [J]. Bulletin of Botanical Research, 2016, 36(3): 380-387.
[14]	LIU Ying；LI De-Wen；MENG Qing-Huan；ZHANG Zhong-Hua；DUAN Xi-Hua. Studies on Dynamic Change of Alkaloids Contents and Expression of the Associated Biosynthetic Genes in Catharathus roseus* Leaves [J]. Bulletin of Botanical Research, 2015, 35(5): 746-750.
[15]	SONG Xin；DONG Jing-Xiang；LI Kai-Long；LI Hong-Jiao；LI Hui-Yu. Cloning and Identification of a Novel thioredoxin h Gene from Tamarix hisipida [J]. Bulletin of Botanical Research, 2015, 35(3): 340-346.

Tumor Cell Absorption of Folate Conjugated Albumin Nanoparticle and Folate Receptor-related Gene Expression

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments