Abstract: Nostoc flagelliforme, a terrestrial cyanobacterium, is distributed in arid and semi-arid steppes of the west and northwest of China. Cyanobacterial NADPH dehydrogenase (NDH-1) is an important photosynthetic membrane protein complex, and is essential to CO₂ uptake, cyclic electron transport around photosystem Ⅰ and cellular respiration. However, little is know about the function roles of NdhK subunit in cyanobacteria. In this study, the ndhK gene was PCR amplified from the N.flagelliforme, and the expression plasmid pET32a-ndhK was generated and transformed into BL21(DE3)pLysS, and the expression of NdhK protein was induced by IPTG. After purification, the fusion protein pET-NdhK was used to immunize Japanese white rabbit to obtain the polyclonal antibody. The titer of the polyclonal antibody was detected and analyzed by Western blotting, the result showed that the polyclonal antibody possessed a high specificity. Therefore, the antibody of NdhK obtained in this study will further help us to reveal the functional roles of cyanobacterial NdhK subunit.

Key words: Nostoc flagelliforme, NdhK protein, polyclonal antibody, protein expression