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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (5): 579-584.doi: 10.7525/j.issn.1673-5102.2011.05.012

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Preliminary Study on In vitro Conservation of Dioscorea bulbifera L. Virus-free Plantlet Germplasm and its Genetic Stability and Virus Change

YIN Ming-Hua;HONG Sen-Rong*   

  1. College of Life Sciences,Shangrao Normal University,Shangrao 334001
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20
  • Contact: HONG Sen-Rong
  • Supported by:

Abstract: The effect of inorganic salts level, sucrose, mannitol and plant growth inhibitor PP333 on In vitro conservation of Dioscorea bulbifera L. virus-free plantlet stems with a bud in MS basic medium was studied using single-factor experiments and orthogonal experimental method. The results showed that the survival rate of D.bulbifera L. virus-free plantlet stems with a bud which cultured on 1/4MS medium containing 2.0 mg·L-1 KT, 0.5 mg·L-1 NAA, 30 g·L-1 sucrose, 20 g·L-1 mannitol and 4 mg·L-1 PP333 and stored at 25±1℃ temperature was over 90%. After morphological indexes measurement, physiological and biochemical indexes determination and RT-PCR analysis, the genetic variation and PVY of In vitro conservation D.bulbifera L. virus-free plantlets were not detected. The results provided a simple and effective way for In vitro conservation of D.bulbifera L. virus-free plantlets germplasm.

Key words: Dioscorea bulbifera L., virus-free plantlets, germplasm, In vitro conservation

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