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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (5): 550-557.doi: 10.7525/j.issn.1673-5102.2011.05.007

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Expressional Analysis of MvNHX1 in Medicago varia Xinmu-1 and Enhancing the Salt Tolerance of Tobacco

ZHANG Hua;ZHANG Fu-Chun*;ZHANG Yu-Liang;ZHANG Bo;CHEN Quan-Jian   

  1. 1.Xinjiang Key Laboratory of Grassland Resources and Ecology,Xinjiang Agricultural University,Urumqi　830052；2.Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University,Urumqi　830052；3.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi　830046；4.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou　571101
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20
  • Contact: ZHANG Fu-Chun
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Abstract: The total RNA of Medicago varia xinmu-1 was extracted, the cDNA fragment amplified by RT-PCR using special primer was linked into pMD19-T vector and transformed into Escherichia coli DH5α. By sequencing the positive clone, the MvNHX1(EU375310) is 1 626 bp long. RT-PCR and real-time PCR assays showed that the level of MvNHX1 transcription was up-regulated and reached a steady higher level in the seedlings after high salinity treatment. The MvNHX1 gene was transformed into tobacco via agrobacterium mediation after the plant expression vectors pBI121-MvNHX1 was successfully constructed. The germination rate and biomass of the transgenic tobacco were taller than those of the control group under NaCl stress, indicating that the MvNHX1 can enhance the salt tolerance of transgenic tobacco.

Key words: Medicago varia xinmu-1, MvNHX1, clone, sequence, salt tolerance

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