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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (3): 313-317.doi: 10.7525/j.issn.1673-5102.2011.03.011

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Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi

ZHENG Gui-Ling;LI Peng   

  1. School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621000
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
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Abstract: Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper. The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction. A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T, including AAPT1 gene controlled by CaMV35s promoter in this experiment. Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way.

Key words: AAPT1 gene, RNAi, plant transformation vector

CLC Number: