Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 603-606.doi: 10.7525/j.issn.1673-5102.2009.05.017
YU Wen-Jing;ZOU Chuan-Shan;WANG Zhi-Ying*;ZHANG Guo-Cai
Using the agrobacterium-mediated approach, the anti-insect gene (the chimeric genes of spider insecticidal and Bt) was successfully transformed into the acceptor of Populus nigra×Populus deltoids “108” as explant. Through PCR analysis, the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes; Through PCRSouthern blots analysis, the result showed that the positive rate was 50%, and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids “108” plants successfully.
Populus deltoids &ldquo,
the chimeric genes of spider insecticidal and Bt,
PCR-Southern blots analysis
YU Wen-Jing;ZOU Chuan-Shan;WANG Zhi-Ying*;ZHANG Guo-Cai. Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt[J]. Bulletin of Botanical Research, 2009, 29(5): 603-606.
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