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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 577-584.doi: 10.7525/j.issn.1673-5102.2009.05.013

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Cloning and Sequence Anaylisis of δ-OAT Gene from Erianthus arundinaceus

WU Yang;HE Li;LI Wei;ZHANG Mu-Qing*   

  1. (1.Key Lab of Ecophysiology and Genetic Improvement for Sugarcane,Ministry of Agricuture,Fujian Agriculture and Forestry University,Fuzhou350002) (2.School of Life Sciences,Jinggangshan University,Ji’an343009)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: ZHANG Mu-Qing
  • Supported by:

Abstract: The complete cDNA sequence of orn-δ-aminotransferase (OAT) gene was obtained from Erianthus arundinaceus and was cloned by reverse-transcript-polymerase-chain-reaction (RT-PCR) and rapid amplification of cDNA end (RACE) technologies. The acquired gene was 1 680 bp in full length, encoding 454 amino acid residues. The amino acid sequence blast results showed that compared to that from mammal, higher plant and microorganism, δ-OAT gene from E.arundinaceus shared the highest homology (87%) with relative genera plant, Saccharum officinarum, 70% homology with other higher plants and 60% homology with animal. No N-terminal mitochondrial transit peptide (MTP) was found in the amino acid sequence encoded by δ-OAT gene from E.arundinaceus, which was the same as that from S.officinarum. Complete domain of OAT, rocD, was included in δ-OAT gene from E.arundinaceus. Expression level of δ-OAT gene from E.arundinaceus treated with 30% polyethylene glycol (PEG) was studied using real-time PCR technology, which showed that after treated 12 hours with PEG, the expression level reached the highest, 4.1 times as that of the comparison, but got lower after stressed 2 hours.

Key words: Erianthus arundinaceus, orn-&delta, -aminotransferase, orn pathway, real-time PCR

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