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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (2): 187-192.doi: 10.7525/j.issn.1673-5102.2009.02.011

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Tissue Culture and Plant Regeneration of Endangered Plant Davidia involucrate Baill

ZOU Li-Juan;SU Zhi-Xian*;HU Jin-Yao;WU Qing-Gui;SU Rui-Jun;YU A-Mei;   

  1. (1.Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation,China West Normal University,Nanchong 637002) (2.Sichuan Provincial Key Laboratory of Ecology and Environmental Technology,Mianyang 621000)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: SU Zhi-Xian
  • Supported by:

Abstract: Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated: the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1; the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1; the medium for rooting culture was White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1, under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation.

Key words: Davidia involucrate Baill, winter bud, clump shoot, shoot segment multiplying, plant regeneration

CLC Number: