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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (6): 767-769.doi: 10.7525/j.issn.1673-5102.2008.06.026

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Determination of Lutein in Leaves of Sweet Potato by HPLC

SHI Jin;LUAN Yu-Shi;*;WANG Jing-Yun;TIAN Jun-Ping   

  1. (1.School of Environmental and Biological Science & Technology,Dalian University of Technology,Dalian 116023)(2.Key Laboratory of Physiology and Ecology of Cold Region Crops,Ministry of Agriculture,Harbin 150030)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20
  • Contact: LUAN Yu-Shi
  • Supported by:

Abstract: A HPLC method for the determination of lutein in leaves of sweet potato was developed in this research for selecting the breed which has the highest content of lutein. The analysis was carried on a Waters SunFireTM C18(150 mm×4.6 mm,5 μm) column. The mobile phase was methanol-water (95:5, v/v), the flow-rate was 0.8 mL·min-1 and wavelength was 445 nm. Linearity was found in the range of 5~100 μg·mL-1, the average recovery was 99.5%, and the relative standard deviations for lutein was 1.9%(n=5). This method is reliable, accurate and suitable for the determination of lutein in leaves of sweet potato. There were significant differences in the lutein content of most samples tested and among them, Sushu No.8 are the highest.

Key words: lutein, sweet potato, HPLC

CLC Number: