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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (6): 705-709.doi: 10.7525/j.issn.1673-5102.2008.06.013

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Cloning and Analysis of hmgr Gene conserved fragments in Euphorbia pekinensis Rupr

CAO Xiao-Ying;JIANG Ji-Hong*;JU Xiu-Yun;DAI Chuan-Chao   

  1. (1.Xuzhou Normal University,Key Laboratory of Biotechnology for Medicinal Plant,Xuzhou 221116) (2.College of Life Sciences,Nanjing Normal University,Nanjing 210097)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20
  • Contact: JIANG Ji-Hong
  • Supported by:

Abstract: Based on total RNA extracted from Euphorbia pekinensis, a 458 bp fragment of hmgr was obtained using reverse transcription PCR strategy and two degenerated oligonucleotides. Sequence analysis revealed that the conserved fragments are 458 bp. At the same time, the three fragments named hmgr1,hmgr2 and hmgr3 were obtained, which were 98.03%、96.29% and 78.38% identification in nucleotide acid compared with hmgr in E.pekinensis reported previously, and 98.68%, 96.71% and 85.53% identification in corresponding amino acid. The three fragments may be new members of hmgr gene family. Sequencing analysis also showed that the three framents had high identity with hmgr from other plants. Deduced amino acid sequence were also analyzed by PROSITE, ClustalX and Phylogenic tree, and data indicated that hmgr3 were different from hmgr1, hmgr2 and hmgr in E.pekinensis reported previously.

Key words: Euphorbia pekinensis Rupr, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), gene cloning

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